Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Xiang An Li; Koichi Kokame; Kousaku Okubo; Kentaro Shimokado; Yoshitane Tsukamoto; Toshiyuki Miyata; Hisao Kato; Chikao Yutani

doi:10.1016/S0167-4781(99)00206-7

Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Xiang An Li, Koichi Kokame, Kousaku Okubo, Kentaro Shimokado, Yoshitane Tsukamoto, Toshiyuki Miyata, Hisao Kato, Chikao Yutani

Physiology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	405-412
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1489
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(99)00206-7
State	Published - Dec 23 1999

Bibliographical note

Funding Information:
The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).

Funding

The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).

Funders	Funder number
Organization for Pharmaceutical Safety and Research of Japan
Japan Science and Technology Agency

Keywords

Human
KRAB
Zinc finger protein
cDNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(99)00206-7

Cite this

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title = "Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers",

abstract = "This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Human, KRAB, Zinc finger protein, cDNA",

author = "Li, \{Xiang An\} and Koichi Kokame and Kousaku Okubo and Kentaro Shimokado and Yoshitane Tsukamoto and Toshiyuki Miyata and Hisao Kato and Chikao Yutani",

note = "Funding Information: The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).",

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doi = "10.1016/S0167-4781(99)00206-7",

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AU - Li, Xiang An

AU - Kokame, Koichi

AU - Okubo, Kousaku

AU - Shimokado, Kentaro

AU - Tsukamoto, Yoshitane

AU - Miyata, Toshiyuki

AU - Kato, Hisao

AU - Yutani, Chikao

N1 - Funding Information: The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).

PY - 1999/12/23

Y1 - 1999/12/23

N2 - This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

AB - This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

KW - Human

KW - KRAB

KW - Zinc finger protein

KW - cDNA

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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ER -

Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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