TY - JOUR
T1 - Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation
AU - Feng, Zhehui
AU - Hong, Jau Shyong
AU - Qi, Qiping
AU - Han, Yifan
AU - Wilson, Belinda
AU - Iadarola, Michael
AU - Tiao, Ningyi
AU - Bing, Guoying
N1 - Funding Information:
This study was supported by a grant from US Army Medical Research (98229027 to GB).
PY - 2000/12/22
Y1 - 2000/12/22
N2 - C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.
AB - C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.
KW - Cloning
KW - Fos-related antigen
KW - Gel-shift
KW - Gene
KW - Guanosine triphosphate
KW - Immunoscreening
KW - Transcription factors
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U2 - 10.1016/S0304-3940(00)01641-4
DO - 10.1016/S0304-3940(00)01641-4
M3 - Article
C2 - 11108998
AN - SCOPUS:0034704402
SN - 0304-3940
VL - 296
SP - 129
EP - 132
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2-3
ER -