Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop-P-methyl resistance in Digitaria sanguinalis

Xiangying Liu, Zhenlin Hou, Yuying Zhang, Austin Merchant, Mei e. Zhong, Guolan Ma, Qing Zeng, Lamei Wu, Xuguo Zhou, Kun Luo, Chunxia Ding

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: Haloxyfop-P-methyl, an acetyl-CoA carboxylase (ACCase)-inhibiting herbicide, has been extensively used to control grass weeds. Widespread use of haloxyfop-P-methyl in cotton fields in China has led to the development of glutathione transferase (GST)-mediated resistance in Digitaria sanguinalis. An RNA-seq analysis identified DsGSTU1, a tau class glutathione transferase from the D. sanguinalis transcriptome as a potential candidate. Here, we cloned DsGSTU1 from D. sanguinalis young leaf tissues and subsequently characterized DsGSTU1 by a combination of sequence analysis, as well as functional heterologous expression in rice. RESULTS: The full-length coding DNA sequence (CDS) of DsGSTU1 is 717 bp in length. Higher DsGSTU1 expression was observed in haloxyfop-P-methyl-resistant (HR) D. sanguinalis than in haloxyfop-P-methyl-susceptible (HS) plants. Overexpression of the DsGSTU1 gene was confirmed by transformation into the wild-type (WT) Nipponbare rice with pBWA(V)HS, a recombinant expression vector. GST activity in transgenic rice seedlings was 1.18–1.40-fold higher than the WT rice seedlings before and after haloxyfop-P-methyl treatment, respectively. Additionally, transgenic rice seedlings overexpressing DsGSTU1 were less sensitive to haloxyfop-P-methyl. CONCLUSION: Our combined findings suggest that DsGSTU1 is involved in metabolic resistance to haloxyfop-P-methyl in D. sanguinalis. A better understanding of the major genes contributing to herbicide-resistant D. sanguinalis facilitates the development of resistance management strategies for this global invasive grass weed.

Original languageEnglish
Pages (from-to)3950-3958
Number of pages9
JournalPest Management Science
Volume79
Issue number10
DOIs
StatePublished - Oct 2023

Bibliographical note

Publisher Copyright:
© 2023 Society of Chemical Industry.

Keywords

  • Digitaria sanguinalis
  • gene expression
  • glutathione transferase
  • haloxyfop-P-methyl
  • metabolic resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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