Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

Debasish Ghosh; Robert E. Brooks; Renyuan Wang; Judith Lesnaw; Michael M. Goodin

doi:10.1016/j.virusres.2008.02.003

Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

Debasish Ghosh, Robert E. Brooks, Renyuan Wang, Judith Lesnaw, Michael M. Goodin

Plant Pathology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We have cloned and characterized mRNAs corresponding to the phosphoprotein (P) and nucleocapsid (N) genes of the sanguinolenta strain of Potato yellow dwarf virus (PYDV). The P and N messenger RNAs both begin with a common AAACA pentanucleotide and are 1546 nt and 962 nt in length, and capable of encoding 52 kDa and 31 kDa proteins, respectively. The N mRNA contains a 12 nt 5′ non-translated sequence (NTS) and a 83 nt 3′-NTS. Similarly, the P mRNA has a 19 nt 5′-NTS and a 125 nt 3′-NTS. Primary structure analyses revealed three potential phosphorylation sites in the P protein and six in the N protein. Despite a lack of predictable nuclear localization signals (NLSs) in either protein, transient expression of the P and N proteins in N. benthamiana showed that both proteins are targeted exclusively to nuclei. Phylogenetic analyses showed that PYDV is most closely related to Maize mosaic virus and Taro vein chlorosis virus, which also lack predictable NLSs in their N proteins. The present data further distinguish PYDV from SYNV and suggest that, together, these viruses serve to provide a more comprehensive view of rhabdovirus cell biology, which can be studied in a common host plant.

Original language	English
Pages (from-to)	26-35
Number of pages	10
Journal	Virus Research
Volume	135
Issue number	1
DOIs	https://doi.org/10.1016/j.virusres.2008.02.003
State	Published - Jul 2008

Bibliographical note

Funding Information:
We sincerely thank Drs. Andrew Jackson and Hei-Ti Tsu for the gift of polyclonal antibodies for the strain-specific detection of PYDV. This research was supported by USDA and Kentucky Tobacco Research and Development Center awards to M.G. This manuscript is published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal Article #08-12-037. Upon request, all novel materials described in this publication will be made available in a timely manner for non-commercial research purposes, subject to the requisite permission from any third-party owners of all or parts of the material. Obtaining any necessary permission will be the responsibility of the requestor.

Keywords

Nicotiana benthamiana
Potato yellow dwarf virus
Sonchus yellow net virus
pSITE vectors

ASJC Scopus subject areas

Cancer Research
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2008.02.003

Cite this

@article{f095688d9ced4aeab55e7c0d5dd1e33c,

title = "Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus",

abstract = "We have cloned and characterized mRNAs corresponding to the phosphoprotein (P) and nucleocapsid (N) genes of the sanguinolenta strain of Potato yellow dwarf virus (PYDV). The P and N messenger RNAs both begin with a common AAACA pentanucleotide and are 1546 nt and 962 nt in length, and capable of encoding 52 kDa and 31 kDa proteins, respectively. The N mRNA contains a 12 nt 5′ non-translated sequence (NTS) and a 83 nt 3′-NTS. Similarly, the P mRNA has a 19 nt 5′-NTS and a 125 nt 3′-NTS. Primary structure analyses revealed three potential phosphorylation sites in the P protein and six in the N protein. Despite a lack of predictable nuclear localization signals (NLSs) in either protein, transient expression of the P and N proteins in N. benthamiana showed that both proteins are targeted exclusively to nuclei. Phylogenetic analyses showed that PYDV is most closely related to Maize mosaic virus and Taro vein chlorosis virus, which also lack predictable NLSs in their N proteins. The present data further distinguish PYDV from SYNV and suggest that, together, these viruses serve to provide a more comprehensive view of rhabdovirus cell biology, which can be studied in a common host plant.",

keywords = "Nicotiana benthamiana, Potato yellow dwarf virus, Sonchus yellow net virus, pSITE vectors",

author = "Debasish Ghosh and Brooks, {Robert E.} and Renyuan Wang and Judith Lesnaw and Goodin, {Michael M.}",

note = "Funding Information: We sincerely thank Drs. Andrew Jackson and Hei-Ti Tsu for the gift of polyclonal antibodies for the strain-specific detection of PYDV. This research was supported by USDA and Kentucky Tobacco Research and Development Center awards to M.G. This manuscript is published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal Article #08-12-037. Upon request, all novel materials described in this publication will be made available in a timely manner for non-commercial research purposes, subject to the requisite permission from any third-party owners of all or parts of the material. Obtaining any necessary permission will be the responsibility of the requestor.",

year = "2008",

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language = "English",

volume = "135",

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T1 - Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

AU - Ghosh, Debasish

AU - Brooks, Robert E.

AU - Wang, Renyuan

AU - Lesnaw, Judith

AU - Goodin, Michael M.

N1 - Funding Information: We sincerely thank Drs. Andrew Jackson and Hei-Ti Tsu for the gift of polyclonal antibodies for the strain-specific detection of PYDV. This research was supported by USDA and Kentucky Tobacco Research and Development Center awards to M.G. This manuscript is published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal Article #08-12-037. Upon request, all novel materials described in this publication will be made available in a timely manner for non-commercial research purposes, subject to the requisite permission from any third-party owners of all or parts of the material. Obtaining any necessary permission will be the responsibility of the requestor.

PY - 2008/7

Y1 - 2008/7

N2 - We have cloned and characterized mRNAs corresponding to the phosphoprotein (P) and nucleocapsid (N) genes of the sanguinolenta strain of Potato yellow dwarf virus (PYDV). The P and N messenger RNAs both begin with a common AAACA pentanucleotide and are 1546 nt and 962 nt in length, and capable of encoding 52 kDa and 31 kDa proteins, respectively. The N mRNA contains a 12 nt 5′ non-translated sequence (NTS) and a 83 nt 3′-NTS. Similarly, the P mRNA has a 19 nt 5′-NTS and a 125 nt 3′-NTS. Primary structure analyses revealed three potential phosphorylation sites in the P protein and six in the N protein. Despite a lack of predictable nuclear localization signals (NLSs) in either protein, transient expression of the P and N proteins in N. benthamiana showed that both proteins are targeted exclusively to nuclei. Phylogenetic analyses showed that PYDV is most closely related to Maize mosaic virus and Taro vein chlorosis virus, which also lack predictable NLSs in their N proteins. The present data further distinguish PYDV from SYNV and suggest that, together, these viruses serve to provide a more comprehensive view of rhabdovirus cell biology, which can be studied in a common host plant.

AB - We have cloned and characterized mRNAs corresponding to the phosphoprotein (P) and nucleocapsid (N) genes of the sanguinolenta strain of Potato yellow dwarf virus (PYDV). The P and N messenger RNAs both begin with a common AAACA pentanucleotide and are 1546 nt and 962 nt in length, and capable of encoding 52 kDa and 31 kDa proteins, respectively. The N mRNA contains a 12 nt 5′ non-translated sequence (NTS) and a 83 nt 3′-NTS. Similarly, the P mRNA has a 19 nt 5′-NTS and a 125 nt 3′-NTS. Primary structure analyses revealed three potential phosphorylation sites in the P protein and six in the N protein. Despite a lack of predictable nuclear localization signals (NLSs) in either protein, transient expression of the P and N proteins in N. benthamiana showed that both proteins are targeted exclusively to nuclei. Phylogenetic analyses showed that PYDV is most closely related to Maize mosaic virus and Taro vein chlorosis virus, which also lack predictable NLSs in their N proteins. The present data further distinguish PYDV from SYNV and suggest that, together, these viruses serve to provide a more comprehensive view of rhabdovirus cell biology, which can be studied in a common host plant.

KW - Nicotiana benthamiana

KW - Potato yellow dwarf virus

KW - Sonchus yellow net virus

KW - pSITE vectors

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M3 - Article

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AN - SCOPUS:44349084779

SN - 0168-1702

VL - 135

SP - 26

EP - 35

JO - Virus Research

JF - Virus Research

IS - 1

ER -

Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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