Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

Debasish Ghosh, Robert E. Brooks, Renyuan Wang, Judith Lesnaw, Michael M. Goodin

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We have cloned and characterized mRNAs corresponding to the phosphoprotein (P) and nucleocapsid (N) genes of the sanguinolenta strain of Potato yellow dwarf virus (PYDV). The P and N messenger RNAs both begin with a common AAACA pentanucleotide and are 1546 nt and 962 nt in length, and capable of encoding 52 kDa and 31 kDa proteins, respectively. The N mRNA contains a 12 nt 5′ non-translated sequence (NTS) and a 83 nt 3′-NTS. Similarly, the P mRNA has a 19 nt 5′-NTS and a 125 nt 3′-NTS. Primary structure analyses revealed three potential phosphorylation sites in the P protein and six in the N protein. Despite a lack of predictable nuclear localization signals (NLSs) in either protein, transient expression of the P and N proteins in N. benthamiana showed that both proteins are targeted exclusively to nuclei. Phylogenetic analyses showed that PYDV is most closely related to Maize mosaic virus and Taro vein chlorosis virus, which also lack predictable NLSs in their N proteins. The present data further distinguish PYDV from SYNV and suggest that, together, these viruses serve to provide a more comprehensive view of rhabdovirus cell biology, which can be studied in a common host plant.

Original languageEnglish
Pages (from-to)26-35
Number of pages10
JournalVirus Research
Volume135
Issue number1
DOIs
StatePublished - Jul 2008

Bibliographical note

Funding Information:
We sincerely thank Drs. Andrew Jackson and Hei-Ti Tsu for the gift of polyclonal antibodies for the strain-specific detection of PYDV. This research was supported by USDA and Kentucky Tobacco Research and Development Center awards to M.G. This manuscript is published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal Article #08-12-037. Upon request, all novel materials described in this publication will be made available in a timely manner for non-commercial research purposes, subject to the requisite permission from any third-party owners of all or parts of the material. Obtaining any necessary permission will be the responsibility of the requestor.

Keywords

  • Nicotiana benthamiana
  • Potato yellow dwarf virus
  • Sonchus yellow net virus
  • pSITE vectors

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus'. Together they form a unique fingerprint.

Cite this