Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts

Charaf Benarafa; Fiona M. Cunningham; Anne S. Hamblin; David W. Horohov; Margaret E. Collins

doi:10.1016/S0165-2427(00)00222-1

Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts

Charaf Benarafa, Fiona M. Cunningham, Anne S. Hamblin, David W. Horohov, Margaret E. Collins

Veterinary Science

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We report the cloning of four equine CC chemokines, eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, which show high levels of identity with their respective homologous sequences in other species. Using a multiplex RT-PCR, we have studied the constitutive mRNA expression of these four CC chemokines in skin, lung, liver, spleen, jejunum, colon and kidney of normal adult horses and compared this data with the eosinophil counts in the same samples. We demonstrate that eotaxin mRNA is only expressed in jejunum and colon, where there are large numbers of eosinophils suggesting that eotaxin might be recruiting eosinophils in the normal digestive tract of the horse. MCP-1 and MCP-4 are expressed in all tissues whereas MCP-2 is only found in some samples of lung, spleen, liver and kidney. We also report the early induction (2h) of equine eotaxin and MCP-4, and the up-regulation of MCP-1 by interleukin-4 in dermal fibroblasts, suggesting these chemokines might be involved in equine skin allergic diseases. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	283-298
Number of pages	16
Journal	Veterinary Immunology and Immunopathology
Volume	76
Issue number	3-4
DOIs	https://doi.org/10.1016/S0165-2427(00)00222-1
State	Published - Oct 31 2000

Bibliographical note

Funding Information:
The authors are very grateful to Kim Francis for her excellent technical assistance with the histology sections and to Prof. David Horohov for the generous gift of IL-4 containing CHO supernatant. We would like to thank Prof. Peter D. Chantler for his helpful comments on the manuscript. This work was supported by the Home of Rest for Horses.

Funding

The authors are very grateful to Kim Francis for her excellent technical assistance with the histology sections and to Prof. David Horohov for the generous gift of IL-4 containing CHO supernatant. We would like to thank Prof. Peter D. Chantler for his helpful comments on the manuscript. This work was supported by the Home of Rest for Horses.

Funders	Funder number
Home of Rest for Horses

Keywords

Chemokine
Eosinophil
Eotaxin
Horse
Interleukin-4

ASJC Scopus subject areas

Immunology
General Veterinary

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10.1016/S0165-2427(00)00222-1

Cite this

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title = "Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts",

abstract = "We report the cloning of four equine CC chemokines, eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, which show high levels of identity with their respective homologous sequences in other species. Using a multiplex RT-PCR, we have studied the constitutive mRNA expression of these four CC chemokines in skin, lung, liver, spleen, jejunum, colon and kidney of normal adult horses and compared this data with the eosinophil counts in the same samples. We demonstrate that eotaxin mRNA is only expressed in jejunum and colon, where there are large numbers of eosinophils suggesting that eotaxin might be recruiting eosinophils in the normal digestive tract of the horse. MCP-1 and MCP-4 are expressed in all tissues whereas MCP-2 is only found in some samples of lung, spleen, liver and kidney. We also report the early induction (2h) of equine eotaxin and MCP-4, and the up-regulation of MCP-1 by interleukin-4 in dermal fibroblasts, suggesting these chemokines might be involved in equine skin allergic diseases. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Chemokine, Eosinophil, Eotaxin, Horse, Interleukin-4",

author = "Charaf Benarafa and Cunningham, {Fiona M.} and Hamblin, {Anne S.} and Horohov, {David W.} and Collins, {Margaret E.}",

note = "Funding Information: The authors are very grateful to Kim Francis for her excellent technical assistance with the histology sections and to Prof. David Horohov for the generous gift of IL-4 containing CHO supernatant. We would like to thank Prof. Peter D. Chantler for his helpful comments on the manuscript. This work was supported by the Home of Rest for Horses.",

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Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts. / Benarafa, Charaf; Cunningham, Fiona M.; Hamblin, Anne S. et al.
In: Veterinary Immunology and Immunopathology, Vol. 76, No. 3-4, 31.10.2000, p. 283-298.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts

AU - Benarafa, Charaf

AU - Cunningham, Fiona M.

AU - Hamblin, Anne S.

AU - Horohov, David W.

AU - Collins, Margaret E.

N1 - Funding Information: The authors are very grateful to Kim Francis for her excellent technical assistance with the histology sections and to Prof. David Horohov for the generous gift of IL-4 containing CHO supernatant. We would like to thank Prof. Peter D. Chantler for his helpful comments on the manuscript. This work was supported by the Home of Rest for Horses.

PY - 2000/10/31

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N2 - We report the cloning of four equine CC chemokines, eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, which show high levels of identity with their respective homologous sequences in other species. Using a multiplex RT-PCR, we have studied the constitutive mRNA expression of these four CC chemokines in skin, lung, liver, spleen, jejunum, colon and kidney of normal adult horses and compared this data with the eosinophil counts in the same samples. We demonstrate that eotaxin mRNA is only expressed in jejunum and colon, where there are large numbers of eosinophils suggesting that eotaxin might be recruiting eosinophils in the normal digestive tract of the horse. MCP-1 and MCP-4 are expressed in all tissues whereas MCP-2 is only found in some samples of lung, spleen, liver and kidney. We also report the early induction (2h) of equine eotaxin and MCP-4, and the up-regulation of MCP-1 by interleukin-4 in dermal fibroblasts, suggesting these chemokines might be involved in equine skin allergic diseases. Copyright (C) 2000 Elsevier Science B.V.

AB - We report the cloning of four equine CC chemokines, eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, which show high levels of identity with their respective homologous sequences in other species. Using a multiplex RT-PCR, we have studied the constitutive mRNA expression of these four CC chemokines in skin, lung, liver, spleen, jejunum, colon and kidney of normal adult horses and compared this data with the eosinophil counts in the same samples. We demonstrate that eotaxin mRNA is only expressed in jejunum and colon, where there are large numbers of eosinophils suggesting that eotaxin might be recruiting eosinophils in the normal digestive tract of the horse. MCP-1 and MCP-4 are expressed in all tissues whereas MCP-2 is only found in some samples of lung, spleen, liver and kidney. We also report the early induction (2h) of equine eotaxin and MCP-4, and the up-regulation of MCP-1 by interleukin-4 in dermal fibroblasts, suggesting these chemokines might be involved in equine skin allergic diseases. Copyright (C) 2000 Elsevier Science B.V.

KW - Chemokine

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KW - Horse

KW - Interleukin-4

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VL - 76

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JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 3-4

ER -

Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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