We report the cloning of four equine CC chemokines, eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, which show high levels of identity with their respective homologous sequences in other species. Using a multiplex RT-PCR, we have studied the constitutive mRNA expression of these four CC chemokines in skin, lung, liver, spleen, jejunum, colon and kidney of normal adult horses and compared this data with the eosinophil counts in the same samples. We demonstrate that eotaxin mRNA is only expressed in jejunum and colon, where there are large numbers of eosinophils suggesting that eotaxin might be recruiting eosinophils in the normal digestive tract of the horse. MCP-1 and MCP-4 are expressed in all tissues whereas MCP-2 is only found in some samples of lung, spleen, liver and kidney. We also report the early induction (2h) of equine eotaxin and MCP-4, and the up-regulation of MCP-1 by interleukin-4 in dermal fibroblasts, suggesting these chemokines might be involved in equine skin allergic diseases. Copyright (C) 2000 Elsevier Science B.V.
|Number of pages||16|
|Journal||Veterinary Immunology and Immunopathology|
|State||Published - Oct 31 2000|
Bibliographical noteFunding Information:
The authors are very grateful to Kim Francis for her excellent technical assistance with the histology sections and to Prof. David Horohov for the generous gift of IL-4 containing CHO supernatant. We would like to thank Prof. Peter D. Chantler for his helpful comments on the manuscript. This work was supported by the Home of Rest for Horses.
ASJC Scopus subject areas
- Veterinary (all)