Co-infection of TYLCV and ToCV increases cathepsin B and promotes ToCV transmission by Bemisia tabaci MED

Ding Yi Hui Lu, Jin Yu Liao, Anugerah Fajar, Jian Bin Chen, Yan Wei, Zhan Hong Zhang, Zhuo Zhang, Li Min Zheng, Xin Qiu Tan, Xu Guo Zhou, Xiao Bin Shi, Yong Liu, De Yong Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Tomato disease is an important disease affecting agricultural production, and the combined infection of tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) has gradually expanded in recent years, but no effective control method has been developed to date. Both viruses are transmitted by Bemisia tabaci Mediteranean (MED). Previously, we found that after B. tabaci MED was fed on ToCV-and TYLCV-infected plants, the transmission efficiency of ToCV was significantly higher than that on plants infected only with ToCV. Therefore, we hypothesize that co-infection could enhance the transmission rates of the virus. In this study, transcriptome sequencing was performed to compare the changes of related transcription factors in B. tabaci MED co-infected with ToCV and TYLCV and infected only with ToCV. Hence, transmission experiments were carried out using B. tabaci MED to clarify the role of cathepsin in virus transmission. The gene expression level and enzyme activity of cathepsin B (Cath B) in B. tabaci MED co-infected with ToCV and TYLCV increased compared with those under ToCV infection alone. After the decrease in cathepsin activity in B. tabaci MED or cathepsin B was silenced, its ability to acquire and transmit ToCV was significantly reduced. We verified the hypothesis that the relative expression of cathepsin B was reduced, which helped reduce ToCV transmission by B. tabaci MED. Therefore, it was speculated that cathepsin has profound research significance in the control of B. tabaci MED and the spread of viral diseases.

Original languageEnglish
Article number1107038
JournalFrontiers in Microbiology
Volume14
DOIs
StatePublished - 2023

Bibliographical note

Publisher Copyright:
Copyright © 2023 Lu, Liao, Fajar, Chen, Wei, Zhang, Zhang, Zheng, Tan, Zhou, Shi, Liu and Zhang.

Keywords

  • Bemisia tabaci
  • co-infection
  • single infection
  • tomato chlorosis virus
  • tomato yellow leaf curl virus

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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