TY - JOUR
T1 - Co-infection of TYLCV and ToCV increases cathepsin B and promotes ToCV transmission by Bemisia tabaci MED
AU - Lu, Ding Yi Hui
AU - Liao, Jin Yu
AU - Fajar, Anugerah
AU - Chen, Jian Bin
AU - Wei, Yan
AU - Zhang, Zhan Hong
AU - Zhang, Zhuo
AU - Zheng, Li Min
AU - Tan, Xin Qiu
AU - Zhou, Xu Guo
AU - Shi, Xiao Bin
AU - Liu, Yong
AU - Zhang, De Yong
N1 - Publisher Copyright:
Copyright © 2023 Lu, Liao, Fajar, Chen, Wei, Zhang, Zhang, Zheng, Tan, Zhou, Shi, Liu and Zhang.
PY - 2023
Y1 - 2023
N2 - Tomato disease is an important disease affecting agricultural production, and the combined infection of tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) has gradually expanded in recent years, but no effective control method has been developed to date. Both viruses are transmitted by Bemisia tabaci Mediteranean (MED). Previously, we found that after B. tabaci MED was fed on ToCV-and TYLCV-infected plants, the transmission efficiency of ToCV was significantly higher than that on plants infected only with ToCV. Therefore, we hypothesize that co-infection could enhance the transmission rates of the virus. In this study, transcriptome sequencing was performed to compare the changes of related transcription factors in B. tabaci MED co-infected with ToCV and TYLCV and infected only with ToCV. Hence, transmission experiments were carried out using B. tabaci MED to clarify the role of cathepsin in virus transmission. The gene expression level and enzyme activity of cathepsin B (Cath B) in B. tabaci MED co-infected with ToCV and TYLCV increased compared with those under ToCV infection alone. After the decrease in cathepsin activity in B. tabaci MED or cathepsin B was silenced, its ability to acquire and transmit ToCV was significantly reduced. We verified the hypothesis that the relative expression of cathepsin B was reduced, which helped reduce ToCV transmission by B. tabaci MED. Therefore, it was speculated that cathepsin has profound research significance in the control of B. tabaci MED and the spread of viral diseases.
AB - Tomato disease is an important disease affecting agricultural production, and the combined infection of tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) has gradually expanded in recent years, but no effective control method has been developed to date. Both viruses are transmitted by Bemisia tabaci Mediteranean (MED). Previously, we found that after B. tabaci MED was fed on ToCV-and TYLCV-infected plants, the transmission efficiency of ToCV was significantly higher than that on plants infected only with ToCV. Therefore, we hypothesize that co-infection could enhance the transmission rates of the virus. In this study, transcriptome sequencing was performed to compare the changes of related transcription factors in B. tabaci MED co-infected with ToCV and TYLCV and infected only with ToCV. Hence, transmission experiments were carried out using B. tabaci MED to clarify the role of cathepsin in virus transmission. The gene expression level and enzyme activity of cathepsin B (Cath B) in B. tabaci MED co-infected with ToCV and TYLCV increased compared with those under ToCV infection alone. After the decrease in cathepsin activity in B. tabaci MED or cathepsin B was silenced, its ability to acquire and transmit ToCV was significantly reduced. We verified the hypothesis that the relative expression of cathepsin B was reduced, which helped reduce ToCV transmission by B. tabaci MED. Therefore, it was speculated that cathepsin has profound research significance in the control of B. tabaci MED and the spread of viral diseases.
KW - Bemisia tabaci
KW - co-infection
KW - single infection
KW - tomato chlorosis virus
KW - tomato yellow leaf curl virus
UR - http://www.scopus.com/inward/record.url?scp=85152067737&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85152067737&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2023.1107038
DO - 10.3389/fmicb.2023.1107038
M3 - Article
AN - SCOPUS:85152067737
SN - 1664-302X
VL - 14
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1107038
ER -