Coding RNA sequencing of equine endometrium during maternal recognition of pregnancy

Kristin M. Klohonatz, Stephen J. Coleman, Alma D. Islas-Trejo, Juan F. Medrano, Ann M. Hess, Ted Kalbfleisch, Milton G. Thomas, Gerrit J. Bouma, Jason E. Bruemmer

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Equine maternal recognition of pregnancy (MRP) is a process whose signal remains unknown. During MRP the conceptus and endometrium communicate to attenuate prostaglandin F (PGF) secretion, sparing the corpus luteum and maintaining progesterone production. Recognition of a mobile conceptus by the endometrium is critical by days 14-16 post-ovulation (PO), when endometrium produces PGF, initiating luteolysis. The objective of this study was to evaluate endometrial gene expression changes based upon pregnancy status via RNA sequencing. This experiment utilized a cross-over design with each mare serving as both a pregnant and non-mated control on days nine, 11, and 13 PO (n = 3/status/day). Mares were randomly assigned to collection day and pregnancy confirmed by terminal uterine lavage at the time of endometrial biopsy. Total RNA was isolated and libraries prepared using Illumina TruSeq RNA sample preparation kit. Reads were mapped and annotated using HISAT2 and Stringtie. Expression values were evaluated with DESEQ2 (P ≤ 0.05 indicated significance). On day nine, 11, and 13 there were 1435, 1435 and 916 significant transcripts, respectively. Multiple genes with splice variants had different expression patterns within the same day. These are the first data to evaluate the endometrial transcriptome during MRP on days nine, 11, and 13.

Original languageEnglish
Article number749
JournalGenes
Volume10
Issue number10
DOIs
StatePublished - Oct 2019

Bibliographical note

Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Equine
  • Maternal recognition of pregnancy
  • Pregnancy
  • Transcriptome

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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