Coleopteran-specific StaufenC functions like Drosophila melanogaster Loquacious-PD in dsRNA processing

Kyungbo Kim; Jinmo Koo; June Sun Yoon; Subba Reddy Palli

doi:10.1080/15476286.2021.1960687

Coleopteran-specific StaufenC functions like Drosophila melanogaster Loquacious-PD in dsRNA processing

Kyungbo Kim
, Jinmo Koo
, June Sun Yoon
, Subba Reddy Palli

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In Drosophila melanogaster, PD isoform of the double-stranded RNA binding protein (dsRBP) Loquacious (Loqs-PD) facilitates dsRNA cleavage to siRNA by Dicer-2. StaufenC (StauC) was discovered as a coleopteran-specific dsRBP required for dsRNA processing in coleopteran insects. Here, we show that StauC is essential for the high RNAi efficiency observed in coleopterans. Knockdown of StauC but not the homologs of Loqs-PD and R2D2 evoked a long-lasting insensitivity to RNAi in the coleopteran cell line, Ledp-SL1. The dsRNA insensitivity induced by StauC knockdown could not be overcome merely by an increase in dose or time of exposure to dsRNA or expression of Loquacious or R2D2. Furthermore, StauC but not Loqs and R2D2 are required for processing of dsRNA into siRNA. StauC overexpression also partly restored the impaired RNAi caused by the knockdown of Loqs-PD in D. melanogaster Kc cells. However, StauC was unable to compensate for the loss-of-the function of Dcr-2 or R2D2. Overall, these data suggest that StauC functions like Lops-PD in processing dsRNA to siRNA.

Original language	English
Pages (from-to)	467-477
Number of pages	11
Journal	RNA Biology
Volume	18
Issue number	S1
DOIs	https://doi.org/10.1080/15476286.2021.1960687
State	Published - 2021

Bibliographical note

Publisher Copyright:
© 2021 Informa UK Limited, trading as Taylor & Francis Group.

Funding

The research is supported by grants from NIH (GM070559‐15 and 1R21AI131427‐01), the NSF (Industry/University Cooperative Research Centers, the Center for Arthropod Management Technologies under Grant IIP‐1821936), Agriculture and Food Research Initiative Competitive Grant No. 2019‐67013‐29351 and the National Institute of Food and Agriculture, U.S. Department of Agriculture (under HATCH Project 2353057000).

Funders	Funder number
National Science Foundation Arctic Social Science Program
National Institutes of Health (NIH)	GM070559‐15
National Institute of Allergy and Infectious F32-AI286447 Cydney N. Johnson Diseases National Institute of Allergy and Infectious R01AI168214 Jason W. Rosch Diseases National Institute of Allergy and Infectious P30 Cydney N. Johnson Diseases National Institute of Allergy and Infectious R00-AI166116 Christopher D. Radka Diseases National Institute of Allergy and Infectious T32-AI106700 Cydney N. Johnson Diseases National Institute of Allergy and Infectious R01AI192221 Jason W. Rosch Diseases National Inst...	R21AI131427
U.S. Department of Agriculture	2353057000
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative
Center for Arthropod Management Technologies	IIP‐1821936, 2019‐67013‐29351

Keywords

Dicer-2
Loqs-PD
RNAi
StaufenC
dsRNA
siRNA

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1080/15476286.2021.1960687

Cite this

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title = "Coleopteran-specific StaufenC functions like Drosophila melanogaster Loquacious-PD in dsRNA processing",

abstract = "In Drosophila melanogaster, PD isoform of the double-stranded RNA binding protein (dsRBP) Loquacious (Loqs-PD) facilitates dsRNA cleavage to siRNA by Dicer-2. StaufenC (StauC) was discovered as a coleopteran-specific dsRBP required for dsRNA processing in coleopteran insects. Here, we show that StauC is essential for the high RNAi efficiency observed in coleopterans. Knockdown of StauC but not the homologs of Loqs-PD and R2D2 evoked a long-lasting insensitivity to RNAi in the coleopteran cell line, Ledp-SL1. The dsRNA insensitivity induced by StauC knockdown could not be overcome merely by an increase in dose or time of exposure to dsRNA or expression of Loquacious or R2D2. Furthermore, StauC but not Loqs and R2D2 are required for processing of dsRNA into siRNA. StauC overexpression also partly restored the impaired RNAi caused by the knockdown of Loqs-PD in D. melanogaster Kc cells. However, StauC was unable to compensate for the loss-of-the function of Dcr-2 or R2D2. Overall, these data suggest that StauC functions like Lops-PD in processing dsRNA to siRNA.",

keywords = "Dicer-2, Loqs-PD, RNAi, StaufenC, dsRNA, siRNA",

author = "Kyungbo Kim and Jinmo Koo and Yoon, \{June Sun\} and \{Reddy Palli\}, Subba",

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year = "2021",

doi = "10.1080/15476286.2021.1960687",

language = "English",

volume = "18",

pages = "467--477",

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T1 - Coleopteran-specific StaufenC functions like Drosophila melanogaster Loquacious-PD in dsRNA processing

AU - Kim, Kyungbo

AU - Koo, Jinmo

AU - Yoon, June Sun

AU - Reddy Palli, Subba

PY - 2021

Y1 - 2021

N2 - In Drosophila melanogaster, PD isoform of the double-stranded RNA binding protein (dsRBP) Loquacious (Loqs-PD) facilitates dsRNA cleavage to siRNA by Dicer-2. StaufenC (StauC) was discovered as a coleopteran-specific dsRBP required for dsRNA processing in coleopteran insects. Here, we show that StauC is essential for the high RNAi efficiency observed in coleopterans. Knockdown of StauC but not the homologs of Loqs-PD and R2D2 evoked a long-lasting insensitivity to RNAi in the coleopteran cell line, Ledp-SL1. The dsRNA insensitivity induced by StauC knockdown could not be overcome merely by an increase in dose or time of exposure to dsRNA or expression of Loquacious or R2D2. Furthermore, StauC but not Loqs and R2D2 are required for processing of dsRNA into siRNA. StauC overexpression also partly restored the impaired RNAi caused by the knockdown of Loqs-PD in D. melanogaster Kc cells. However, StauC was unable to compensate for the loss-of-the function of Dcr-2 or R2D2. Overall, these data suggest that StauC functions like Lops-PD in processing dsRNA to siRNA.

AB - In Drosophila melanogaster, PD isoform of the double-stranded RNA binding protein (dsRBP) Loquacious (Loqs-PD) facilitates dsRNA cleavage to siRNA by Dicer-2. StaufenC (StauC) was discovered as a coleopteran-specific dsRBP required for dsRNA processing in coleopteran insects. Here, we show that StauC is essential for the high RNAi efficiency observed in coleopterans. Knockdown of StauC but not the homologs of Loqs-PD and R2D2 evoked a long-lasting insensitivity to RNAi in the coleopteran cell line, Ledp-SL1. The dsRNA insensitivity induced by StauC knockdown could not be overcome merely by an increase in dose or time of exposure to dsRNA or expression of Loquacious or R2D2. Furthermore, StauC but not Loqs and R2D2 are required for processing of dsRNA into siRNA. StauC overexpression also partly restored the impaired RNAi caused by the knockdown of Loqs-PD in D. melanogaster Kc cells. However, StauC was unable to compensate for the loss-of-the function of Dcr-2 or R2D2. Overall, these data suggest that StauC functions like Lops-PD in processing dsRNA to siRNA.

KW - Dicer-2

KW - Loqs-PD

KW - RNAi

KW - StaufenC

KW - dsRNA

KW - siRNA

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VL - 18

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JO - RNA Biology

JF - RNA Biology

IS - S1

ER -

Coleopteran-specific StaufenC functions like Drosophila melanogaster Loquacious-PD in dsRNA processing

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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