Combination of C17 Sphingoid Base Homologues and Mass Spectrometry Analysis as a New Approach to Study Sphingolipid Metabolism

Stefka Spassieva, Jacek Bielawski, Viviana Anelli, Lina M. Obeid

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

30 Scopus citations

Abstract

In recent years, sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate have emerged as important second messengers in addition to their role as precursors of biomembrane components. The investigation of these sphingolipid metabolites requires the development of new, more sensitive methods for assaying the enzymes involved in their production. This chapter describes the utilization of mass spectrometry technology in combination with nonnaturally occurring C17 sphingoid bases in the in vitro assays of two of the enzymes of the sphingolipid pathway, ceramide synthase and sphingosine kinase. These new in vitro methods provide high sensitivity and extreme accuracy even when crude extracts are used as enzyme sources.

Original languageEnglish
Title of host publicationLipidomics and Bioactive Lipids
Subtitle of host publicationLipids and Cell Signaling
EditorsAlex Brown
Pages233-241
Number of pages9
DOIs
StatePublished - 2007

Publication series

NameMethods in Enzymology
Volume434
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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