Combination of C17 Sphingoid Base Homologues and Mass Spectrometry Analysis as a New Approach to Study Sphingolipid Metabolism

Stefka Spassieva; Jacek Bielawski; Viviana Anelli; Lina M. Obeid

doi:10.1016/S0076-6879(07)34012-3

Combination of C₁₇ Sphingoid Base Homologues and Mass Spectrometry Analysis as a New Approach to Study Sphingolipid Metabolism

Stefka Spassieva, Jacek Bielawski, Viviana Anelli, Lina M. Obeid

Physiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

33 Scopus citations

Abstract

In recent years, sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate have emerged as important second messengers in addition to their role as precursors of biomembrane components. The investigation of these sphingolipid metabolites requires the development of new, more sensitive methods for assaying the enzymes involved in their production. This chapter describes the utilization of mass spectrometry technology in combination with nonnaturally occurring C₁₇ sphingoid bases in the in vitro assays of two of the enzymes of the sphingolipid pathway, ceramide synthase and sphingosine kinase. These new in vitro methods provide high sensitivity and extreme accuracy even when crude extracts are used as enzyme sources.

Original language	English
Title of host publication	Lipidomics and Bioactive Lipids
Subtitle of host publication	Lipids and Cell Signaling
Editors	Alex Brown
Pages	233-241
Number of pages	9
DOIs	https://doi.org/10.1016/S0076-6879(07)34012-3
State	Published - 2007

Publication series

Name	Methods in Enzymology
Volume	434
ISSN (Print)	0076-6879

Funding

Funders	Funder number
National Childhood Cancer Registry – National Cancer Institute	P01CA097132

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1016/S0076-6879(07)34012-3

Cite this

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title = "Combination of C17 Sphingoid Base Homologues and Mass Spectrometry Analysis as a New Approach to Study Sphingolipid Metabolism",

abstract = "In recent years, sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate have emerged as important second messengers in addition to their role as precursors of biomembrane components. The investigation of these sphingolipid metabolites requires the development of new, more sensitive methods for assaying the enzymes involved in their production. This chapter describes the utilization of mass spectrometry technology in combination with nonnaturally occurring C17 sphingoid bases in the in vitro assays of two of the enzymes of the sphingolipid pathway, ceramide synthase and sphingosine kinase. These new in vitro methods provide high sensitivity and extreme accuracy even when crude extracts are used as enzyme sources.",

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Combination of C₁₇ Sphingoid Base Homologues and Mass Spectrometry Analysis as a New Approach to Study Sphingolipid Metabolism. / Spassieva, Stefka; Bielawski, Jacek; Anelli, Viviana et al.
Lipidomics and Bioactive Lipids: Lipids and Cell Signaling. ed. / Alex Brown. 2007. p. 233-241 (Methods in Enzymology; Vol. 434).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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