Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses

María Pérez; Felipe Lombó; Irfan Baig; Alfredo F. Braña; Jürgen Rohr; José A. Salas; Carmen Méndez

doi:10.1128/AEM.01266-06

Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses

María Pérez, Felipe Lombó, Irfan Baig, Alfredo F. Braña, Jürgen Rohr, José A. Salas, Carmen Méndez

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Combinatorial biosynthesis was applied to Streptomyces deoxysugar biosynthesis genes in order to reconstitute "unnatural natural gene clusters" for the biosynthesis of four D-deoxysugars (D-olivose, D-oliose, D-digitoxose, and D-boivinose). Expression of these gene clusters in Streptomyces albus 16F4 was used to prove the functionality of the designed clusters through the generation of glycosylated tetracenomycins. Three glycosylated tetracenomycins were generated and characterized, two of which (D-digitoxosyl-tetracenomycin C and D-boivinosyl-tetracenocmycin C) were novel compounds. The constructed gene clusters may be used to increase the capabilities of microorganisms to synthesize new deoxysugars and therefore to produce new glycosylated bioactive compounds.

Original language	English
Pages (from-to)	6644-6652
Number of pages	9
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	10
DOIs	https://doi.org/10.1128/AEM.01266-06
State	Published - Oct 2006

Funding

Funders	Funder number
National Childhood Cancer Registry – National Cancer Institute	R01CA091901

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Food Science
Biotechnology
Ecology

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10.1128/AEM.01266-06

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Pérez, M., Lombó, F., Baig, I., Braña, A. F., Rohr, J., Salas, J. A., & Méndez, C. (2006). Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses. Applied and Environmental Microbiology, 72(10), 6644-6652. https://doi.org/10.1128/AEM.01266-06

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abstract = "Combinatorial biosynthesis was applied to Streptomyces deoxysugar biosynthesis genes in order to reconstitute {"}unnatural natural gene clusters{"} for the biosynthesis of four D-deoxysugars (D-olivose, D-oliose, D-digitoxose, and D-boivinose). Expression of these gene clusters in Streptomyces albus 16F4 was used to prove the functionality of the designed clusters through the generation of glycosylated tetracenomycins. Three glycosylated tetracenomycins were generated and characterized, two of which (D-digitoxosyl-tetracenomycin C and D-boivinosyl-tetracenocmycin C) were novel compounds. The constructed gene clusters may be used to increase the capabilities of microorganisms to synthesize new deoxysugars and therefore to produce new glycosylated bioactive compounds.",

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Pérez, M, Lombó, F, Baig, I, Braña, AF, Rohr, J, Salas, JA & Méndez, C 2006, 'Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses', Applied and Environmental Microbiology, vol. 72, no. 10, pp. 6644-6652. https://doi.org/10.1128/AEM.01266-06

Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses. / Pérez, María; Lombó, Felipe; Baig, Irfan et al.
In: Applied and Environmental Microbiology, Vol. 72, No. 10, 10.2006, p. 6644-6652.

Research output: Contribution to journal › Article › peer-review

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Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses

Abstract

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