Combined effect of MP0, GSTM1 and GSTT1 polymorphisms on chromosome aberrations and lung cancer risk

Nohelia Cajas-Salazar, Carlos H. Sierra-Torres, Salama A. Salama, Joseph B. Zwischenberger, William W. Au

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The role of myeloperoxidase (MPO), and glutathione S-transferase μ and θ (GSTM1 and GSTT1) genetic polymorphisms on lung cancer risk was investigated in 110 Caucasian patients and 119 matched controls. Single genotype variants were not significantly associated with lung cancer risk. However, inheritance of the combined GSTM1 and GSTT1 null genotypes showed a significant increase in risk (crude OR = 2.32, 95% CI=1.01-6.04). Based on adjustment by age, gender and smoking history, the MPO GA interacted with the presence of GSTM1 and GSTT1 genotypes to significantly reduce the risk (OR=0.17, 95% CI = 0.03-0.98). From the chromosome aberration (CA) study in a subgroup of 79 patients and 69 matched controls, patients had significantly more CA than the controls. Among the patients, GSTM1 null was associated with a significant increase of CA and MPO AA was associated with a significant decrease of CA compared to their respective wild-type genotypes. After stratifying by smoking history (≤ and > 40 pack-years) and genotype, patients still had significantly more CA than the respective controls in most genotype categories. This indicates that the patients had additional contributing factors such as other susceptibility genes and/or different styles of smoking compared with the controls. In conclusion, our study indicates that CA is a useful biomarker to show the functional characteristics of genotypes and the interactive effects from combined genotypes. Therefore, our study strengthens the combined use of genotype and biomarkers for genetic susceptibility to environmental cancer.

Original languageEnglish
Pages (from-to)473-483
Number of pages11
JournalInternational Journal of Hygiene and Environmental Health
Issue number6
StatePublished - Oct 2003

Bibliographical note

Funding Information:
Acknowledgements. This study was partially supported by a grant from the NIEHS Center Grant (#ES06676) to the University of Texas Medical Branch (UTMB) and Colciencias-Fulbright-LASPAU fellowships to N. C. S. and to C. H. S. T. The study utilized services from the General Clinical Research Center (GCRC) with funding from the National Center for Research Resources (M01 RR-00073), and services from the Molecular Biology Core of the NIEHS Center, both at UTMB.


  • Chromosome aberrations
  • Cigarette smoking
  • Glutathione S-transferase M1
  • Glutathione S-transferase T1
  • Lung cancer
  • Myeloperoxidase

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health


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