TY - JOUR
T1 - Combined Effects of a Metabolic Inhibitor (Gabaculine) and an Uptake Inhibitor (Ketamine) on the γ‐Aminobutyrate System in Mouse Brain
AU - Wood, J. D.
AU - Geddes, J. W.
AU - Tsui, S. ‐K
AU - Kurylo, E.
PY - 1982/12
Y1 - 1982/12
N2 - Abstract: The intramuscular administration of a γ‐aminobutyrate‐α‐oxoglutarate aminotransferase (GABA‐T) inhibitor, gabaculine, to mice resulted in significant increases in GABA content and decreases in the content of aspartate, glutamate, and glutamine in the nerve endings (synaptosomes). These effects were ameliorated by the concurrent administration of the GABA uptake inhibitor ketamine. A major cause of these effects was the gabaculine‐induced inhibition of GABA‐T activity and the lessening of this inhibition by ketamine. The latter phenomenon was not due to a direct action of ketamine on the enzyme, nor to an interaction between gabaculine and ketamine. Rather, it appeared that ketamine might be interfering with the transport of gabaculine into the cellular structures. The anticonvulsant action of the GABA‐T inhibitor and the GABA uptake inhibitor together was little different from that of the GABA‐T inhibitor alone.
AB - Abstract: The intramuscular administration of a γ‐aminobutyrate‐α‐oxoglutarate aminotransferase (GABA‐T) inhibitor, gabaculine, to mice resulted in significant increases in GABA content and decreases in the content of aspartate, glutamate, and glutamine in the nerve endings (synaptosomes). These effects were ameliorated by the concurrent administration of the GABA uptake inhibitor ketamine. A major cause of these effects was the gabaculine‐induced inhibition of GABA‐T activity and the lessening of this inhibition by ketamine. The latter phenomenon was not due to a direct action of ketamine on the enzyme, nor to an interaction between gabaculine and ketamine. Rather, it appeared that ketamine might be interfering with the transport of gabaculine into the cellular structures. The anticonvulsant action of the GABA‐T inhibitor and the GABA uptake inhibitor together was little different from that of the GABA‐T inhibitor alone.
KW - Convulsions
KW - Gabaculine
KW - Ketamine
KW - γ‐Aminobutyrate uptake
KW - γ‐Aminobutyrate‐α‐oxoglutarate aminotransferase
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U2 - 10.1111/j.1471-4159.1982.tb08007.x
DO - 10.1111/j.1471-4159.1982.tb08007.x
M3 - Article
C2 - 7142997
AN - SCOPUS:0020363006
SN - 0022-3042
VL - 39
SP - 1710
EP - 1715
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 6
ER -