Comparative analysis of double-stranded RNA degradation and processing in insects

Indrakant K. Singh; Satnam Singh; Kanakachari Mogilicherla; Jayendra Nath Shukla; Subba Reddy Palli

doi:10.1038/s41598-017-17134-2

Comparative analysis of double-stranded RNA degradation and processing in insects

Indrakant K. Singh
, Satnam Singh
, Kanakachari Mogilicherla
, Jayendra Nath Shukla
, Subba Reddy Palli

Research output: Contribution to journal › Article › peer-review

204 Scopus citations

Abstract

RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within the same order. We identified homologs of key RNAi genes in the genomes of some of these insects and studied their domain architecture. These data suggest that dsRNA digestion by dsRNases and its processing to siRNAs in the cells are among the major factors contributing to differential RNAi efficiency reported among insects.

Original language	English
Article number	17059
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-17134-2
State	Published - Dec 1 2017

Bibliographical note

Publisher Copyright:
© 2017 The Author(s).

Funding

This work was supported by National Institutes of Health (1R21AI131427-01) and by the National Institute of Food and Agriculture, USDA, HATCH under 2351177000. This is publication number 17-08-103 from the Kentucky Agricultural Experimental Station and is published with the approval of the director. We thank Dr. Ric Bessin for help with collection and identification of insects; Dr. Amit Roy and Mr. June-Sun Yoon for help with preparation of Figures. I.K.S. and S.S. were supported by UGC India Raman Fellowship.

Funders	Funder number
National Institutes of Health (NIH)	1R21AI131427-01
National Institutes of Health (NIH)
U.S. Department of Agriculture	2351177000
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative
Research Grants Council, University Grants Committee

ASJC Scopus subject areas

General

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10.1038/s41598-017-17134-2

Cite this

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abstract = "RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within the same order. We identified homologs of key RNAi genes in the genomes of some of these insects and studied their domain architecture. These data suggest that dsRNA digestion by dsRNases and its processing to siRNAs in the cells are among the major factors contributing to differential RNAi efficiency reported among insects.",

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Comparative analysis of double-stranded RNA degradation and processing in insects

Abstract

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