Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources

Charles E. Wyman; Venkatesh Balan; Bruce E. Dale; Richard T. Elander; Matthew Falls; Bonnie Hames; Mark T. Holtzapple; Michael R. Ladisch; Y. Y. Lee; Nathan Mosier; Venkata R. Pallapolu; Jian Shi; Steven R. Thomas; Ryan E. Warner

doi:10.1016/j.biortech.2011.06.069

Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources

Charles E. Wyman, Venkatesh Balan, Bruce E. Dale, Richard T. Elander, Matthew Falls, Bonnie Hames, Mark T. Holtzapple, Michael R. Ladisch, Y. Y. Lee, Nathan Mosier, Venkata R. Pallapolu, Jian Shi, Steven R. Thomas, Ryan E. Warner

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

Dilute sulfuric acid (DA), sulfur dioxide (SO ₂), liquid hot water (LHW), soaking in aqueous ammonia (SAA), ammonia fiber expansion (AFEX), and lime pretreatments were applied to Alamo, Dacotah, and Shawnee switchgrass. Application of the same analytical methods and material balance approaches facilitated meaningful comparisons of glucose and xylose yields from combined pretreatment and enzymatic hydrolysis. Use of a common supply of cellulase, beta-glucosidase, and xylanase also eased comparisons. All pretreatments enhanced sugar recovery from pretreatment and subsequent enzymatic hydrolysis substantially compared to untreated switchgrass. Adding beta-glucosidase was effective early in enzymatic hydrolysis while cellobiose levels were high but had limited effect on longer term yields at the enzyme loadings applied. Adding xylanase improved yields most for higher pH pretreatments where more xylan was left in the solids. Harvest time had more impact on performance than switchgrass variety, and microscopy showed changes in different features could impact performance by different pretreatments.

Original language	English
Pages (from-to)	11052-11062
Number of pages	11
Journal	Bioresource Technology
Volume	102
Issue number	24
DOIs	https://doi.org/10.1016/j.biortech.2011.06.069
State	Published - Dec 2011

Bibliographical note

Funding Information:
Funding by the Office of the Biomass Program of the United States Department of Energy through Contract No. DE-FG36-07GO17102 to the University of California at Riverside was vital to performing this research. The true collaborative spirit of the CAFI Team made this project possible and pleasurable, and we thank the many undergraduate and graduate students, post-doctoral candidates, technicians, administrative assistants, and others on the CAFI Team for their vital role in developing this information. Dr. Rajeev Kumar from the University of California at Riverside provided very thorough and thoughtful reviews of the paper and offered many suggestions and corrections that are greatly appreciated. We also acknowledge that this paper records the final project by the CAFI team that has been together for over 10 years. Finally, the corresponding author would like to thank the Ford Motor Company for funding the Chair in Environmental Engineering that helps make projects such as this possible.

Keywords

Hydrolysis
Microscopy
Pretreatment
Switchgrass
Yields

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2011.06.069

Cite this

Wyman, C. E., Balan, V., Dale, B. E., Elander, R. T., Falls, M., Hames, B., Holtzapple, M. T., Ladisch, M. R., Lee, Y. Y., Mosier, N., Pallapolu, V. R., Shi, J., Thomas, S. R., & Warner, R. E. (2011). Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources. Bioresource Technology, 102(24), 11052-11062. https://doi.org/10.1016/j.biortech.2011.06.069

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title = "Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources",

abstract = "Dilute sulfuric acid (DA), sulfur dioxide (SO 2), liquid hot water (LHW), soaking in aqueous ammonia (SAA), ammonia fiber expansion (AFEX), and lime pretreatments were applied to Alamo, Dacotah, and Shawnee switchgrass. Application of the same analytical methods and material balance approaches facilitated meaningful comparisons of glucose and xylose yields from combined pretreatment and enzymatic hydrolysis. Use of a common supply of cellulase, beta-glucosidase, and xylanase also eased comparisons. All pretreatments enhanced sugar recovery from pretreatment and subsequent enzymatic hydrolysis substantially compared to untreated switchgrass. Adding beta-glucosidase was effective early in enzymatic hydrolysis while cellobiose levels were high but had limited effect on longer term yields at the enzyme loadings applied. Adding xylanase improved yields most for higher pH pretreatments where more xylan was left in the solids. Harvest time had more impact on performance than switchgrass variety, and microscopy showed changes in different features could impact performance by different pretreatments.",

keywords = "Hydrolysis, Microscopy, Pretreatment, Switchgrass, Yields",

author = "Wyman, {Charles E.} and Venkatesh Balan and Dale, {Bruce E.} and Elander, {Richard T.} and Matthew Falls and Bonnie Hames and Holtzapple, {Mark T.} and Ladisch, {Michael R.} and Lee, {Y. Y.} and Nathan Mosier and Pallapolu, {Venkata R.} and Jian Shi and Thomas, {Steven R.} and Warner, {Ryan E.}",

note = "Funding Information: Funding by the Office of the Biomass Program of the United States Department of Energy through Contract No. DE-FG36-07GO17102 to the University of California at Riverside was vital to performing this research. The true collaborative spirit of the CAFI Team made this project possible and pleasurable, and we thank the many undergraduate and graduate students, post-doctoral candidates, technicians, administrative assistants, and others on the CAFI Team for their vital role in developing this information. Dr. Rajeev Kumar from the University of California at Riverside provided very thorough and thoughtful reviews of the paper and offered many suggestions and corrections that are greatly appreciated. We also acknowledge that this paper records the final project by the CAFI team that has been together for over 10 years. Finally, the corresponding author would like to thank the Ford Motor Company for funding the Chair in Environmental Engineering that helps make projects such as this possible.",

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doi = "10.1016/j.biortech.2011.06.069",

language = "English",

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Wyman, CE, Balan, V, Dale, BE, Elander, RT, Falls, M, Hames, B, Holtzapple, MT, Ladisch, MR, Lee, YY, Mosier, N, Pallapolu, VR, Shi, J, Thomas, SR & Warner, RE 2011, 'Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources', Bioresource Technology, vol. 102, no. 24, pp. 11052-11062. https://doi.org/10.1016/j.biortech.2011.06.069

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T1 - Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources

AU - Wyman, Charles E.

AU - Balan, Venkatesh

AU - Dale, Bruce E.

AU - Elander, Richard T.

AU - Falls, Matthew

AU - Hames, Bonnie

AU - Holtzapple, Mark T.

AU - Ladisch, Michael R.

AU - Lee, Y. Y.

AU - Mosier, Nathan

AU - Pallapolu, Venkata R.

AU - Shi, Jian

AU - Thomas, Steven R.

AU - Warner, Ryan E.

N1 - Funding Information: Funding by the Office of the Biomass Program of the United States Department of Energy through Contract No. DE-FG36-07GO17102 to the University of California at Riverside was vital to performing this research. The true collaborative spirit of the CAFI Team made this project possible and pleasurable, and we thank the many undergraduate and graduate students, post-doctoral candidates, technicians, administrative assistants, and others on the CAFI Team for their vital role in developing this information. Dr. Rajeev Kumar from the University of California at Riverside provided very thorough and thoughtful reviews of the paper and offered many suggestions and corrections that are greatly appreciated. We also acknowledge that this paper records the final project by the CAFI team that has been together for over 10 years. Finally, the corresponding author would like to thank the Ford Motor Company for funding the Chair in Environmental Engineering that helps make projects such as this possible.

PY - 2011/12

Y1 - 2011/12

N2 - Dilute sulfuric acid (DA), sulfur dioxide (SO 2), liquid hot water (LHW), soaking in aqueous ammonia (SAA), ammonia fiber expansion (AFEX), and lime pretreatments were applied to Alamo, Dacotah, and Shawnee switchgrass. Application of the same analytical methods and material balance approaches facilitated meaningful comparisons of glucose and xylose yields from combined pretreatment and enzymatic hydrolysis. Use of a common supply of cellulase, beta-glucosidase, and xylanase also eased comparisons. All pretreatments enhanced sugar recovery from pretreatment and subsequent enzymatic hydrolysis substantially compared to untreated switchgrass. Adding beta-glucosidase was effective early in enzymatic hydrolysis while cellobiose levels were high but had limited effect on longer term yields at the enzyme loadings applied. Adding xylanase improved yields most for higher pH pretreatments where more xylan was left in the solids. Harvest time had more impact on performance than switchgrass variety, and microscopy showed changes in different features could impact performance by different pretreatments.

AB - Dilute sulfuric acid (DA), sulfur dioxide (SO 2), liquid hot water (LHW), soaking in aqueous ammonia (SAA), ammonia fiber expansion (AFEX), and lime pretreatments were applied to Alamo, Dacotah, and Shawnee switchgrass. Application of the same analytical methods and material balance approaches facilitated meaningful comparisons of glucose and xylose yields from combined pretreatment and enzymatic hydrolysis. Use of a common supply of cellulase, beta-glucosidase, and xylanase also eased comparisons. All pretreatments enhanced sugar recovery from pretreatment and subsequent enzymatic hydrolysis substantially compared to untreated switchgrass. Adding beta-glucosidase was effective early in enzymatic hydrolysis while cellobiose levels were high but had limited effect on longer term yields at the enzyme loadings applied. Adding xylanase improved yields most for higher pH pretreatments where more xylan was left in the solids. Harvest time had more impact on performance than switchgrass variety, and microscopy showed changes in different features could impact performance by different pretreatments.

KW - Hydrolysis

KW - Microscopy

KW - Pretreatment

KW - Switchgrass

KW - Yields

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Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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