Comparative effects of neurotensin and neuromedin N on growth of human pancreatic cancer, MIA PaCa-2

S. Sumi, B. M. Evers, C. M. Townsend, K. Yoshinaga, T. Uchida, M. Murakami, K. Sato, J. Ishizuka, J. C. Thompson

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Neurotensin (NT), an important regulatory hormone of the gut, stimulates growth of the human pancreatic cancer cell line MIA PaCa-2 in vitro. The purpose of our study was to compare the stimulatory effects of NT and neuromedin N (NMN), a structurally related hexapeptide, on the growth of MIA PaCa-2. In addition, the effects of NT on the growth of MIA PaCa-2 xenografts and normal GI tissues were assessed in athymic nude mice. MIA PaCa-2 cells, plated in serum-free media, were treated with either NT (10-12-10-6M) or NMN (10-11-10-7 m) and cells were counted. For the in vivo study, MIA PaCa-2 cells were inoculated sc into 30 athymic nude mice and then randomized to two groups to receive either NT (600 μg kg-1, sc, tid) or vehicle. At sacrifice (day 35), the xenografted tumours, as well as normal host pancreas, jejunum and ileum were removed, weighed, and assayed for DNA, RNA and protein. Both NT and NMN stimulated the growth of MIA PaCa-2 cells in vitro with maximal (approximately 30%) increases occurring with dosages of 10-9 m. In vivo, NT had a transient effect on xenografted MIA PaCa-2 tumour area with increases noted on days 21 and 25 of the study. Conversely, NT significantly stimulated the growth of jejunum and ileum, with a more pronounced effect noted in the jejunum. NT and NMN have similar growthstimulatory effects on MIA PaCa-2 cells in vitro, which suggests an interaction through the same receptor. In contrast, NT, at a dose that is trophic for the small bowel of mice, failed to stimulate growth of either MIA PaCa-2 xenografts or normal pancreas, suggesting that NT may not play a major role in the growth of the pancreas in vivo.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalSurgical Oncology
Issue number5
StatePublished - Oct 1993

Bibliographical note

Funding Information:
Correspondence: Courtney M. Townsend, Jr., MD, Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555-0533, USA. This study was supported by grants from the National Institutes of Health (NIH) (PO1 DK 35608, 5R37 DK 15241 and R29 AG 10885) and the American Cancer Society (CB-571). tVisiting scientist from the First Department of Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan. ~:Visiting Scientist from the Department of Surgery, Faculty of Medicine, Tokyo Medical and Dental College, Tokyo, Japan. §Visiting Scientist from the Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.


  • cell growth
  • neuromedin N
  • neurotensin
  • pancreatic cancer

ASJC Scopus subject areas

  • Surgery
  • Oncology


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