Comparative proteomic analysis of longissimus dorsi muscle in immuno- and surgically castrated male pigs

Xuebin Shi; Chunbao Li; Miaodan Cao; Xinglian Xu; Guanghong Zhou; Youling L. Xiong

doi:10.1016/j.foodchem.2015.11.059

Comparative proteomic analysis of longissimus dorsi muscle in immuno- and surgically castrated male pigs

Xuebin Shi, Chunbao Li, Miaodan Cao, Xinglian Xu, Guanghong Zhou, Youling L. Xiong

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We compared proteomic profiles of male pig muscles after active immunization against gonadotropin releasing hormone (GnRH) and surgical castration. Longissimus dorsi samples were collected from immunocastration (IC) and surgical castration (SC) groups (n = 15 each). Muscle proteins were extracted and then identified by data-independent label-free nano LC-MS/MS. A total of 610 proteins were identified, 50 of which were differentially expressed (P < 0.05) between immuno- and surgical castration. Twenty-two of 50 differentially expressed proteins were higher in abundance for IC group and 27 proteins with abundance change folds greater than 1.5 differed with castration methods. Proteins involved in cytoskeleton and immunity were abundant in IC group. Several heat shock proteins (HSPs) and laminins were abundant in SC group.

Original language	English
Pages (from-to)	885-892
Number of pages	8
Journal	Food Chemistry
Volume	199
DOIs	https://doi.org/10.1016/j.foodchem.2015.11.059
State	Published - May 15 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

Chaperone
Immunocastration
Laminin
Proteomics
Surgical castration

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2015.11.059

Cite this

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title = "Comparative proteomic analysis of longissimus dorsi muscle in immuno- and surgically castrated male pigs",

abstract = "We compared proteomic profiles of male pig muscles after active immunization against gonadotropin releasing hormone (GnRH) and surgical castration. Longissimus dorsi samples were collected from immunocastration (IC) and surgical castration (SC) groups (n = 15 each). Muscle proteins were extracted and then identified by data-independent label-free nano LC-MS/MS. A total of 610 proteins were identified, 50 of which were differentially expressed (P < 0.05) between immuno- and surgical castration. Twenty-two of 50 differentially expressed proteins were higher in abundance for IC group and 27 proteins with abundance change folds greater than 1.5 differed with castration methods. Proteins involved in cytoskeleton and immunity were abundant in IC group. Several heat shock proteins (HSPs) and laminins were abundant in SC group.",

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author = "Xuebin Shi and Chunbao Li and Miaodan Cao and Xinglian Xu and Guanghong Zhou and Xiong, {Youling L.}",

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AU - Shi, Xuebin

AU - Li, Chunbao

AU - Cao, Miaodan

AU - Xu, Xinglian

AU - Zhou, Guanghong

AU - Xiong, Youling L.

PY - 2016/5/15

Y1 - 2016/5/15

N2 - We compared proteomic profiles of male pig muscles after active immunization against gonadotropin releasing hormone (GnRH) and surgical castration. Longissimus dorsi samples were collected from immunocastration (IC) and surgical castration (SC) groups (n = 15 each). Muscle proteins were extracted and then identified by data-independent label-free nano LC-MS/MS. A total of 610 proteins were identified, 50 of which were differentially expressed (P < 0.05) between immuno- and surgical castration. Twenty-two of 50 differentially expressed proteins were higher in abundance for IC group and 27 proteins with abundance change folds greater than 1.5 differed with castration methods. Proteins involved in cytoskeleton and immunity were abundant in IC group. Several heat shock proteins (HSPs) and laminins were abundant in SC group.

AB - We compared proteomic profiles of male pig muscles after active immunization against gonadotropin releasing hormone (GnRH) and surgical castration. Longissimus dorsi samples were collected from immunocastration (IC) and surgical castration (SC) groups (n = 15 each). Muscle proteins were extracted and then identified by data-independent label-free nano LC-MS/MS. A total of 610 proteins were identified, 50 of which were differentially expressed (P < 0.05) between immuno- and surgical castration. Twenty-two of 50 differentially expressed proteins were higher in abundance for IC group and 27 proteins with abundance change folds greater than 1.5 differed with castration methods. Proteins involved in cytoskeleton and immunity were abundant in IC group. Several heat shock proteins (HSPs) and laminins were abundant in SC group.

KW - Chaperone

KW - Immunocastration

KW - Laminin

KW - Proteomics

KW - Surgical castration

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JO - Food Chemistry

JF - Food Chemistry

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Comparative proteomic analysis of longissimus dorsi muscle in immuno- and surgically castrated male pigs

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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