Comparative time-course of lipid and myofibrillar protein oxidation in different biphasic systems under hydroxyl radical stress

Jiayi Yang, Youling L. Xiong

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

This study aimed to investigate the relative reaction rate of protein and lipid oxidation in different biphasic model systems (linoleic acid; liposome; emulsion) containing myofibrillar protein (MFP at 1, 8 and 20 mg/mL) under hydroxyl radical stress. Protein oxidation markers exhibited significant changes in 2 h: reduced tryptophan fluorescence intensity, carbonyl formation, and extensive polymerization of myosin. In contrast, no detectable changes (P >.05) in lipid peroxide occurred within 2 h except for samples with 1 mg/mL MFP which showed an early TBARS formation. Of the three biphasic systems, the oxidative stability of lipids followed the order of emulsion > linoleic acid > liposome, indicating the steric role of proteins. In general, MFP was more susceptible to radicals than lipids, and a higher protein:lipid ratio was associated with a slower TBARS production and more rapid protein oxidation, suggesting a sacrificing role of MFP to protect lipids.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalFood Chemistry
Volume243
DOIs
StatePublished - Mar 15 2018

Bibliographical note

Publisher Copyright:
© 2017

Keywords

  • Emulsion
  • Linoleic acid
  • Lipid oxidation
  • Liposome
  • Protein oxidation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Fingerprint

Dive into the research topics of 'Comparative time-course of lipid and myofibrillar protein oxidation in different biphasic systems under hydroxyl radical stress'. Together they form a unique fingerprint.

Cite this