Comparison of porcine airway and intestinal epithelial cell lines for the susceptibility and expression of pattern recognition receptors upon influenza virus infection

Milton Thomas; Max Pierson; Tirth Uprety; Laihua Zhu; Zhiguang Ran; Chithra C. Sreenivasan; Dan Wang; Ben Hause; David H. Francis; Feng Li; Radhey S. Kaushik

doi:10.3390/v10060312

Comparison of porcine airway and intestinal epithelial cell lines for the susceptibility and expression of pattern recognition receptors upon influenza virus infection

Milton Thomas, Max Pierson, Tirth Uprety, Laihua Zhu, Zhiguang Ran, Chithra C. Sreenivasan, Dan Wang, Ben Hause, David H. Francis, Feng Li, Radhey S. Kaushik

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Influenza viruses infect the epithelial cells of the swine respiratory tract. Cell lines derived from the respiratory tract of pigs could serve as an excellent in vitro model for studying the pathogenesis of influenza viruses. In this study, we examined the replication of influenza viruses in the MK1-OSU cell line, which was clonally derived from pig airway epithelium. MK1-OSU cells expressed both cytokeratin and vimentin proteins and displayed several sugar moieties on the cell membrane. These cells also expressed both Sial2-3Gal and Sial2-6Gal receptors and were susceptible to swine influenza A, but not to human B and C viruses. Interestingly, these cells were also permissive to infection by influenza D virus that utilized 9-O-acetylated glycans. To study the differences in the expression of pattern recognition receptors (PRRs) upon influenza virus infection in the respiratory and digestive tract, we compared the protein expression of various PRRs in MK1-OSU cells with that in the SD-PJEC cell line, a clonally derived cell line from the porcine jejunal epithelium. Toll-like receptor 7 (TLR-7) and melanoma differentiation-associated protein 5 (MDA5) receptors showed decreased expression in influenza A infected MK1-OSU cells, while only TLR-7 expression decreased in SD-PJEC cells. Further research is warranted to study the mechanism behind the virus-mediated suppression of these proteins. Overall, this study shows that the porcine respiratory epithelial cell line, MK1-OSU, could serve as an in-vitro model for studying the pathogenesis and innate immune responses to porcine influenza viruses.

Original language	English
Article number	312
Journal	Viruses
Volume	10
Issue number	6
DOIs	https://doi.org/10.3390/v10060312
State	Published - Jun 7 2018

Bibliographical note

Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.

Funding

We thank Mahesh Khatri (Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA) for providing the MK1-OSU cell line. Radhey S. Kaushik, Milton Thomas, Max Pierson, Tirth Uprety, and this research project were funded and supported by USDA NIFA SDSU Agricultural Experiment Station Hatch grants # SD00H326-09 and SD00H547-15. This study was also partially funded by NIH R15 grant number 1R15AI089690-01A1. This study was also in part supported by SDSU-AES 4AH-477, USDA/NIFA 2016-67016-24949, NIH R21AI107379, National Science Foundation/EPSCoR (http://www.nsf.gov/od/iia/programs/epscor/index.jsp) award IIA-1335423, and the State of South Dakota’s Governor’s Office of Economic Development as a South Dakota Research Innovation Center. Acknowledgments: We thank Mahesh Khatri (Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA) for providing the MK1-OSU cell line. Radhey S. Kaushik, Milton Thomas, Max Pierson, Tirth Uprety, and this research project were funded and supported by USDA NIFA SDSU Agricultural Experiment Station Hatch grants # SD00H326-09 and SD00H547-15. This study was also partially funded by NIH R15 grant number 1R15AI089690-01A1. This study was also in part supported by SDSU-AES 4AH-477, USDA/NIFA 2016-67016-24949, NIH R21AI107379, National Science Foundation/EPSCoR (http://www.nsf.gov/od/iia/programs/epscor/index.jsp) award IIA-1335423, and the State of South Dakota’s Governor’s Office of Economic Development as a South Dakota Research Innovation Center.

Funders	Funder number
National Science Foundation/EPSCoR
SDSU AES	4AH-477, USDA/NIFA 2016-67016-24949
USDA NIFA SDSU Agricultural Experiment Station Hatch	SD00H326-09, SD00H547-15
National Science Foundation (NSF)
National Institutes of Health (NIH)	R21AI107379
National Institute of Allergy and Infectious Diseases	R15AI089690
U.S. Department of Agriculture
Office of Experimental Program to Stimulate Competitive Research	IIA-1335423
National Institute of Food and Agriculture	2016-67016-24949
San Diego State University
Arizona Agricultural Experiment Station

Keywords

Influenza
Innate immunity
MK1-OSU
RIG-I-like receptor
Respiratory epithelial cells
SD-PJEC
TLR

ASJC Scopus subject areas

Infectious Diseases
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/v10060312

Cite this

Thomas, M., Pierson, M., Uprety, T., Zhu, L., Ran, Z., Sreenivasan, C. C., Wang, D., Hause, B., Francis, D. H., Li, F., & Kaushik, R. S. (2018). Comparison of porcine airway and intestinal epithelial cell lines for the susceptibility and expression of pattern recognition receptors upon influenza virus infection. Viruses, 10(6), Article 312. https://doi.org/10.3390/v10060312

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title = "Comparison of porcine airway and intestinal epithelial cell lines for the susceptibility and expression of pattern recognition receptors upon influenza virus infection",

abstract = "Influenza viruses infect the epithelial cells of the swine respiratory tract. Cell lines derived from the respiratory tract of pigs could serve as an excellent in vitro model for studying the pathogenesis of influenza viruses. In this study, we examined the replication of influenza viruses in the MK1-OSU cell line, which was clonally derived from pig airway epithelium. MK1-OSU cells expressed both cytokeratin and vimentin proteins and displayed several sugar moieties on the cell membrane. These cells also expressed both Sial2-3Gal and Sial2-6Gal receptors and were susceptible to swine influenza A, but not to human B and C viruses. Interestingly, these cells were also permissive to infection by influenza D virus that utilized 9-O-acetylated glycans. To study the differences in the expression of pattern recognition receptors (PRRs) upon influenza virus infection in the respiratory and digestive tract, we compared the protein expression of various PRRs in MK1-OSU cells with that in the SD-PJEC cell line, a clonally derived cell line from the porcine jejunal epithelium. Toll-like receptor 7 (TLR-7) and melanoma differentiation-associated protein 5 (MDA5) receptors showed decreased expression in influenza A infected MK1-OSU cells, while only TLR-7 expression decreased in SD-PJEC cells. Further research is warranted to study the mechanism behind the virus-mediated suppression of these proteins. Overall, this study shows that the porcine respiratory epithelial cell line, MK1-OSU, could serve as an in-vitro model for studying the pathogenesis and innate immune responses to porcine influenza viruses.",

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AU - Sreenivasan, Chithra C.

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AU - Francis, David H.

AU - Li, Feng

AU - Kaushik, Radhey S.

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N2 - Influenza viruses infect the epithelial cells of the swine respiratory tract. Cell lines derived from the respiratory tract of pigs could serve as an excellent in vitro model for studying the pathogenesis of influenza viruses. In this study, we examined the replication of influenza viruses in the MK1-OSU cell line, which was clonally derived from pig airway epithelium. MK1-OSU cells expressed both cytokeratin and vimentin proteins and displayed several sugar moieties on the cell membrane. These cells also expressed both Sial2-3Gal and Sial2-6Gal receptors and were susceptible to swine influenza A, but not to human B and C viruses. Interestingly, these cells were also permissive to infection by influenza D virus that utilized 9-O-acetylated glycans. To study the differences in the expression of pattern recognition receptors (PRRs) upon influenza virus infection in the respiratory and digestive tract, we compared the protein expression of various PRRs in MK1-OSU cells with that in the SD-PJEC cell line, a clonally derived cell line from the porcine jejunal epithelium. Toll-like receptor 7 (TLR-7) and melanoma differentiation-associated protein 5 (MDA5) receptors showed decreased expression in influenza A infected MK1-OSU cells, while only TLR-7 expression decreased in SD-PJEC cells. Further research is warranted to study the mechanism behind the virus-mediated suppression of these proteins. Overall, this study shows that the porcine respiratory epithelial cell line, MK1-OSU, could serve as an in-vitro model for studying the pathogenesis and innate immune responses to porcine influenza viruses.

AB - Influenza viruses infect the epithelial cells of the swine respiratory tract. Cell lines derived from the respiratory tract of pigs could serve as an excellent in vitro model for studying the pathogenesis of influenza viruses. In this study, we examined the replication of influenza viruses in the MK1-OSU cell line, which was clonally derived from pig airway epithelium. MK1-OSU cells expressed both cytokeratin and vimentin proteins and displayed several sugar moieties on the cell membrane. These cells also expressed both Sial2-3Gal and Sial2-6Gal receptors and were susceptible to swine influenza A, but not to human B and C viruses. Interestingly, these cells were also permissive to infection by influenza D virus that utilized 9-O-acetylated glycans. To study the differences in the expression of pattern recognition receptors (PRRs) upon influenza virus infection in the respiratory and digestive tract, we compared the protein expression of various PRRs in MK1-OSU cells with that in the SD-PJEC cell line, a clonally derived cell line from the porcine jejunal epithelium. Toll-like receptor 7 (TLR-7) and melanoma differentiation-associated protein 5 (MDA5) receptors showed decreased expression in influenza A infected MK1-OSU cells, while only TLR-7 expression decreased in SD-PJEC cells. Further research is warranted to study the mechanism behind the virus-mediated suppression of these proteins. Overall, this study shows that the porcine respiratory epithelial cell line, MK1-OSU, could serve as an in-vitro model for studying the pathogenesis and innate immune responses to porcine influenza viruses.

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Comparison of porcine airway and intestinal epithelial cell lines for the susceptibility and expression of pattern recognition receptors upon influenza virus infection

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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Cite this