Comparison of the Promoter Region of the Human and Porcine Choline Acetyltransferase Genes: Localization of an Important Enhancer Region

Louis B. Hersh; Chuang Fong Kong; Craig Sampson; Gabriele Mues; Yi‐Ping ‐P Li; Ann Fisher; Dana Hilt; E. Edward Baetge

doi:10.1111/j.1471-4159.1993.tb03569.x

Comparison of the Promoter Region of the Human and Porcine Choline Acetyltransferase Genes: Localization of an Important Enhancer Region

Louis B. Hersh, Chuang Fong Kong, Craig Sampson, Gabriele Mues, Yi‐Ping ‐P Li, Ann Fisher, Dana Hilt, E. Edward Baetge

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Abstract: Genomic clones of human and porcine choline acetyltransferase were obtained by screening genomic libraries with synthetic oligonucleotides. The human and porcine genes exhibit significant conservation of both their intron/exon structure and the nucleotide sequence in their 5’flanking regions. However, the two genes differ in several respects, including the absence of a “TATA” box in the human gene and differences in the position of the methionine start codon. Analysis of the promoter region of the two genes has led to the localization of an enhancer element that appears necessary for efficient transcription of the gene.

Original language	English
Pages (from-to)	306-314
Number of pages	9
Journal	Journal of Neurochemistry
Volume	61
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-4159.1993.tb03569.x
State	Published - Jul 1993

Keywords

Choline acetyltransferase
Enhancer
Gene regulation
Genomic clones
Transfection analysis

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1993.tb03569.x

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title = "Comparison of the Promoter Region of the Human and Porcine Choline Acetyltransferase Genes: Localization of an Important Enhancer Region",

abstract = "Abstract: Genomic clones of human and porcine choline acetyltransferase were obtained by screening genomic libraries with synthetic oligonucleotides. The human and porcine genes exhibit significant conservation of both their intron/exon structure and the nucleotide sequence in their 5{\textquoteright}flanking regions. However, the two genes differ in several respects, including the absence of a “TATA” box in the human gene and differences in the position of the methionine start codon. Analysis of the promoter region of the two genes has led to the localization of an enhancer element that appears necessary for efficient transcription of the gene.",

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T1 - Comparison of the Promoter Region of the Human and Porcine Choline Acetyltransferase Genes

T2 - Localization of an Important Enhancer Region

AU - Hersh, Louis B.

AU - Kong, Chuang Fong

AU - Sampson, Craig

AU - Mues, Gabriele

AU - Li, Yi‐Ping ‐P

AU - Fisher, Ann

AU - Hilt, Dana

AU - Baetge, E. Edward

PY - 1993/7

Y1 - 1993/7

N2 - Abstract: Genomic clones of human and porcine choline acetyltransferase were obtained by screening genomic libraries with synthetic oligonucleotides. The human and porcine genes exhibit significant conservation of both their intron/exon structure and the nucleotide sequence in their 5’flanking regions. However, the two genes differ in several respects, including the absence of a “TATA” box in the human gene and differences in the position of the methionine start codon. Analysis of the promoter region of the two genes has led to the localization of an enhancer element that appears necessary for efficient transcription of the gene.

AB - Abstract: Genomic clones of human and porcine choline acetyltransferase were obtained by screening genomic libraries with synthetic oligonucleotides. The human and porcine genes exhibit significant conservation of both their intron/exon structure and the nucleotide sequence in their 5’flanking regions. However, the two genes differ in several respects, including the absence of a “TATA” box in the human gene and differences in the position of the methionine start codon. Analysis of the promoter region of the two genes has led to the localization of an enhancer element that appears necessary for efficient transcription of the gene.

KW - Choline acetyltransferase

KW - Enhancer

KW - Gene regulation

KW - Genomic clones

KW - Transfection analysis

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Comparison of the Promoter Region of the Human and Porcine Choline Acetyltransferase Genes: Localization of an Important Enhancer Region

Abstract

Keywords

ASJC Scopus subject areas

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