Comparison of the Promoter Region of the Human and Porcine Choline Acetyltransferase Genes: Localization of an Important Enhancer Region

Louis B. Hersh, Chuang Fong Kong, Craig Sampson, Gabriele Mues, Yi‐Ping ‐P Li, Ann Fisher, Dana Hilt, E. Edward Baetge

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Abstract: Genomic clones of human and porcine choline acetyltransferase were obtained by screening genomic libraries with synthetic oligonucleotides. The human and porcine genes exhibit significant conservation of both their intron/exon structure and the nucleotide sequence in their 5’flanking regions. However, the two genes differ in several respects, including the absence of a “TATA” box in the human gene and differences in the position of the methionine start codon. Analysis of the promoter region of the two genes has led to the localization of an enhancer element that appears necessary for efficient transcription of the gene.

Original languageEnglish
Pages (from-to)306-314
Number of pages9
JournalJournal of Neurochemistry
Volume61
Issue number1
DOIs
StatePublished - Jul 1993

Keywords

  • Choline acetyltransferase
  • Enhancer
  • Gene regulation
  • Genomic clones
  • Transfection analysis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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