Comparison of the structure and expression of the human and rat neprilysin (endopeptidase 24.11)-encoding genes

Chingwen Li; Guojin Chen; Norma P. Gerard; Craig Gerard; Carmen R. Bozic; Louis B. Hersh

doi:10.1016/0378-1119(95)00464-H

Comparison of the structure and expression of the human and rat neprilysin (endopeptidase 24.11)-encoding genes

Chingwen Li, Guojin Chen, Norma P. Gerard, Craig Gerard, Carmen R. Bozic, Louis B. Hersh

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The existence of a third non-coding exon in the human neprilysin-encoding gene (h-NEP), positionally located as exon 3, has been demonstrated by reverse transcription of RNA from human kidney and lung, coupled with the polymerase chain reaction. Comparison of nucleotide sequences between h-NEP and the rat NEP (r-NEP) genes shows a high degree of sequence conservation within noncoding exons 1 and 2. In contrast, the region of the gene containing exon 3 is highly divergent. Two transcripts derived from exon 2 by alternative splicing, type-2a and type-2b, were demonstrated in human kidney and lung. In contrast, only the type-2b transcript was present in these same tissues in the rat. The type-1 transcript was detected in human kidney, lung and brain, this transcript appearing to be the major species in brain.

Original language	English
Pages (from-to)	363-366
Number of pages	4
Journal	Gene
Volume	164
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00464-H
State	Published - Oct 27 1995

Bibliographical note

Funding Information:
This researchw ass upporteidn part by grantD A02243 from the NationalI nstituteo n Drug Abuse and a grant from the Tobaccoa nd Health ResearchIn stituteo f the Universityo f KentuckyW. e thank Mr. Jim Smith for his excellentte chnicaal ssistance.

Keywords

Noncoding exon
alternative splicing
multiple mRNA
tissue-specific expression

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(95)00464-H

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title = "Comparison of the structure and expression of the human and rat neprilysin (endopeptidase 24.11)-encoding genes",

abstract = "The existence of a third non-coding exon in the human neprilysin-encoding gene (h-NEP), positionally located as exon 3, has been demonstrated by reverse transcription of RNA from human kidney and lung, coupled with the polymerase chain reaction. Comparison of nucleotide sequences between h-NEP and the rat NEP (r-NEP) genes shows a high degree of sequence conservation within noncoding exons 1 and 2. In contrast, the region of the gene containing exon 3 is highly divergent. Two transcripts derived from exon 2 by alternative splicing, type-2a and type-2b, were demonstrated in human kidney and lung. In contrast, only the type-2b transcript was present in these same tissues in the rat. The type-1 transcript was detected in human kidney, lung and brain, this transcript appearing to be the major species in brain.",

keywords = "Noncoding exon, alternative splicing, multiple mRNA, tissue-specific expression",

author = "Chingwen Li and Guojin Chen and Gerard, {Norma P.} and Craig Gerard and Bozic, {Carmen R.} and Hersh, {Louis B.}",

note = "Funding Information: This researchw ass upporteidn part by grantD A02243 from the NationalI nstituteo n Drug Abuse and a grant from the Tobaccoa nd Health ResearchIn stituteo f the Universityo f KentuckyW. e thank Mr. Jim Smith for his excellentte chnicaal ssistance.",

year = "1995",

month = oct,

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doi = "10.1016/0378-1119(95)00464-H",

language = "English",

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T1 - Comparison of the structure and expression of the human and rat neprilysin (endopeptidase 24.11)-encoding genes

AU - Li, Chingwen

AU - Chen, Guojin

AU - Gerard, Norma P.

AU - Gerard, Craig

AU - Bozic, Carmen R.

AU - Hersh, Louis B.

N1 - Funding Information: This researchw ass upporteidn part by grantD A02243 from the NationalI nstituteo n Drug Abuse and a grant from the Tobaccoa nd Health ResearchIn stituteo f the Universityo f KentuckyW. e thank Mr. Jim Smith for his excellentte chnicaal ssistance.

PY - 1995/10/27

Y1 - 1995/10/27

N2 - The existence of a third non-coding exon in the human neprilysin-encoding gene (h-NEP), positionally located as exon 3, has been demonstrated by reverse transcription of RNA from human kidney and lung, coupled with the polymerase chain reaction. Comparison of nucleotide sequences between h-NEP and the rat NEP (r-NEP) genes shows a high degree of sequence conservation within noncoding exons 1 and 2. In contrast, the region of the gene containing exon 3 is highly divergent. Two transcripts derived from exon 2 by alternative splicing, type-2a and type-2b, were demonstrated in human kidney and lung. In contrast, only the type-2b transcript was present in these same tissues in the rat. The type-1 transcript was detected in human kidney, lung and brain, this transcript appearing to be the major species in brain.

AB - The existence of a third non-coding exon in the human neprilysin-encoding gene (h-NEP), positionally located as exon 3, has been demonstrated by reverse transcription of RNA from human kidney and lung, coupled with the polymerase chain reaction. Comparison of nucleotide sequences between h-NEP and the rat NEP (r-NEP) genes shows a high degree of sequence conservation within noncoding exons 1 and 2. In contrast, the region of the gene containing exon 3 is highly divergent. Two transcripts derived from exon 2 by alternative splicing, type-2a and type-2b, were demonstrated in human kidney and lung. In contrast, only the type-2b transcript was present in these same tissues in the rat. The type-1 transcript was detected in human kidney, lung and brain, this transcript appearing to be the major species in brain.

KW - Noncoding exon

KW - alternative splicing

KW - multiple mRNA

KW - tissue-specific expression

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AN - SCOPUS:0028883725

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JO - Gene

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Comparison of the structure and expression of the human and rat neprilysin (endopeptidase 24.11)-encoding genes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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