Competitive binding assay for drugs that interfere with calmodulin function

Harry Le Vine, Naji Sahyoun, Pedro Cuatrecasas

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


A direct 125I-calmodulin-binding system utilizing a particulate cytoskeletal preparation of the neuronal calmodulin-dependent protein kinase type II provides a simple, rapid filtration-based method for assessing anti-calmodulin drug activity. The binding assay avoids potential artifacts due to direct drug effects on the standard phosphodiesterase enzyme assay while obtaining comparable IC50 values for a spectrum of drugs. It also provides a tool to probe mechanisms of action of putative anticalmodulin agents which may help elucidate selective pharmacologic modifications of pathophysiological processes.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalAnalytical Biochemistry
Issue number1
StatePublished - Nov 15 1985


  • I-calmodulin
  • cytoskeletal protein kinase
  • filtration

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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