Abstract
A direct 125I-calmodulin-binding system utilizing a particulate cytoskeletal preparation of the neuronal calmodulin-dependent protein kinase type II provides a simple, rapid filtration-based method for assessing anti-calmodulin drug activity. The binding assay avoids potential artifacts due to direct drug effects on the standard phosphodiesterase enzyme assay while obtaining comparable IC50 values for a spectrum of drugs. It also provides a tool to probe mechanisms of action of putative anticalmodulin agents which may help elucidate selective pharmacologic modifications of pathophysiological processes.
Original language | English |
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Pages (from-to) | 153-158 |
Number of pages | 6 |
Journal | Analytical Biochemistry |
Volume | 151 |
Issue number | 1 |
DOIs | |
State | Published - Nov 15 1985 |
Keywords
- I-calmodulin
- cytoskeletal protein kinase
- filtration
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology