Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova; Anton V. Liopo; Valery G. Andreev; Inga Cicenaite; B. Mark Evers; Shilla Chakrabarty; Todd C. Pappas; Rinat O. Esenaliev

doi:10.1016/j.canlet.2007.11.023

Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova, Anton V. Liopo, Valery G. Andreev, Inga Cicenaite, B. Mark Evers, Shilla Chakrabarty, Todd C. Pappas, Rinat O. Esenaliev

Research output: Contribution to journal › Article › peer-review

138 Scopus citations

Abstract

The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

Original language	English
Pages (from-to)	215-225
Number of pages	11
Journal	Cancer Letters
Volume	261
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2007.11.023
State	Published - Mar 18 2008

Bibliographical note

Funding Information:
These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

Funding

These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

Funders	Funder number
Department of Defense prostate cancer research program	81XWH-04-1-0247
National Institutes of Health (NIH)
National Childhood Cancer Registry – National Cancer Institute	R01CA104748

Keywords

Gene delivery
PEI
PLGA nanoparticles
Tumor
Ultrasound

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2007.11.023

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title = "Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo",

abstract = "The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.",

keywords = "Gene delivery, PEI, PLGA nanoparticles, Tumor, Ultrasound",

author = "Chumakova, {Olga V.} and Liopo, {Anton V.} and Andreev, {Valery G.} and Inga Cicenaite and Evers, {B. Mark} and Shilla Chakrabarty and Pappas, {Todd C.} and Esenaliev, {Rinat O.}",

note = "Funding Information: These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748. ",

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doi = "10.1016/j.canlet.2007.11.023",

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AU - Cicenaite, Inga

AU - Evers, B. Mark

AU - Chakrabarty, Shilla

AU - Pappas, Todd C.

AU - Esenaliev, Rinat O.

N1 - Funding Information: These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

PY - 2008/3/18

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N2 - The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

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KW - Ultrasound

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Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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