Composition of the metabolomic bio-coronas isolated from Ocimum sanctum and Rubia tinctorum

Jasmina Kurepa, Jan A. Smalle

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objective: Nanoharvesting from intact plants, organs, and cultured cells is a method in which nanoparticles are co-incubated with the target tissue, which leads to the internalization of nanoparticles. Internalized nanoparticles are coated in situ with specific metabolites that form a dynamic surface layer called a bio-corona. Our previous study showed that metabolites that form the bio-corona around anatase TiO2 nanoparticles incubated with leaves of the model plant Arabidopsis thaliana are enriched for flavonoids and lipids. The present study focused on the identification of metabolites isolated by nanoharvesting from two medicinal plants, Ocimum sanctum (Tulsi) and Rubia tinctorum (common madder). Results: To identify metabolites that form the bio-corona, Tulsi leaves and madder roots were incubated with ultra-small anatase TiO2 nanoparticles, the coated nanoparticles were collected, and the adsorbed molecules were released from the nanoparticle surface and analyzed using an untargeted metabolomics approach. Similar to the results in which Arabidopsis tissue was used as a source of metabolites, TiO2 nanoparticle bio-coronas from Tulsi and madder were enriched for flavonoids and lipids, suggesting that nanoharvesting has a wide-range application potential. The third group of metabolites enriched in bio-coronas isolated from both plants were small peptides with C-terminal arginine and lysine residues.

Original languageEnglish
Article number6
JournalBMC Research Notes
Issue number1
StatePublished - Dec 2021

Bibliographical note

Publisher Copyright:
© 2020, The Author(s).


  • Common madder
  • Flavonoids
  • Holy basil
  • Lipids
  • Ocimum sanctum
  • Rubia tinctorum
  • Titanium dioxide nanoparticles
  • Tulsi

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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