Computational design of a human butyrylcholinesterase mutant for accelerating cocaine hydrolysis based on the transition-state simulation

Daquan Gao, Hoon Cho, Wenchao Yang, Yongmei Pan, Guangfu Yang, Hsin Hsiung Tai, Chang Guo Zhan

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Breaking down cocaine: The transition-state simulation predicts a significantly more stable transition-state structure (see model; C: green, O: red, H: gray, N: blue, cocaine atoms: yellow) for the rate-determining first reaction step (TSI) of the (-)-cocaine hydrolysis catalyzed by a mutant of human butyrylcholinesterase (BChE). (Figure Presented).

Original languageEnglish
Pages (from-to)653-657
Number of pages5
JournalAngewandte Chemie - International Edition
Volume45
Issue number4
DOIs
StatePublished - Jan 16 2006

Keywords

  • Cocaine
  • Enzymes
  • Molecular dynamics
  • Protein engineering
  • Transition states

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)

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