To assess the utility of two temperature-sensitive (ts) mutant vaccinia viruses as vectors for the conditional in vitro expression of recombinant foreign genes, we have studied the kinetics of expression of foreign genes incorporated into these viruses. At nonpermissive temperature, 40°C, these viruses were defective either in DNA synthesis or in virus assembly. Foreign gene expression was affected by the nature of the ts lesion and by the nature of the vaccinia promoter positioned upstream from the foreign gene. With both vector viruses, a foreign gene controlled by the p7.5 early-late promoter was expressed at both 33° and 40°C. With the DNA synthesis-defective vector virus, foreign gene expression controlled by the p11 DNA synthesis-dependent late promoter was inhibited at 40°C, but could be turned on by shift to 33°C. This ts expression system provides an alternative to use of drugs that inhibit DNA synthesis as a means for experimental manipulation of gene expression. Both vector viruses can be used with existing vaccinia virus expression technology.
|Number of pages||4|
|State||Published - Nov 15 1990|
Bibliographical noteFunding Information:
This work was supported by USPHS Contract No. AI-52586 from the National Institutes of Health, and by American Lebanese Syrian Associated Charities. We thank Samuel Dales for providing the ts mutant vaccinia viruses, Bernard Moss for providing the pSCI 1 plasmid, and John M. Freeman for technical assistance.
- DNA synthesis inhibition
- Recombinant DNA
- gene expression vector
- influenza hemagglutinin
- transcription promoter
ASJC Scopus subject areas