Thermo-sensitive genic male sterile (TGMS) rice has a number of desirable characteristics for hybrid rice production. Many studies have demonstrated that the sterility of TGMS rice is controlled by a single recessive gene. It has been mapped for the first time on chromosome 8 and named tms 1. Several AFLP markers which tightly linked to the tms 1 gene have been identified recently. In order to develop a detailed physical map of the tms 1 gene-encompassing region and finally clone the tms 1 gene, a bacterial artificial chromosome (BAC) library of rice 5460F (the fertile mutant line of TGMS rice 5460S) using a modified vector pECBAC1 has been constructed. The constructed 5460F BAC library consists of 16 896 clones with an average insert size of 119 kb, which represents about 4.7 times rice haploid genome equivalents. Neither chloroplast nor mitochondrial DNA was detected from the library. The library was screened with three single copy sequence amplified fragment length polymorphism (AFLP) markers which tightly linked to tms 1 gene as probes and eight positive clones were identified.
|Number of pages||6|
|Journal||Chinese Science Bulletin|
|State||Published - Sep 1999|
- Bacterial artificial chromosome
- Thermo-sensitive genic male sterile
ASJC Scopus subject areas