Construction and characterization of a bacterial artificial chromosome library of peach

Qian Wang; Kaichun Zhang; Xueping Qu; Jianhang Jia; Chuanyou Li; Demin Jin; Bin Wang

Construction and characterization of a bacterial artificial chromosome library of peach

Qian Wang
, Kaichun Zhang
, Xueping Qu
, Jianhang Jia
, Chuanyou Li
, Demin Jin
, Bin Wang

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This report briefly describes the construction and characterization of a peach [prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAG) library. The variety Jingyu has many important agronomic characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clones. The BAG library has an average insert size of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two randomly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach respectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and physical mapping of peach genome.

Original language	English
Pages (from-to)	526-528
Number of pages	3
Journal	Progress in Natural Science
Volume	10
Issue number	7
State	Published - 2000

Keywords

Bacterial artificial chromosome
Genomic library construction
Prunus persica

ASJC Scopus subject areas

General Materials Science

Cite this

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title = "Construction and characterization of a bacterial artificial chromosome library of peach",

abstract = "This report briefly describes the construction and characterization of a peach [prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAG) library. The variety Jingyu has many important agronomic characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clones. The BAG library has an average insert size of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two randomly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach respectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and physical mapping of peach genome.",

keywords = "Bacterial artificial chromosome, Genomic library construction, Prunus persica",

author = "Qian Wang and Kaichun Zhang and Xueping Qu and Jianhang Jia and Chuanyou Li and Demin Jin and Bin Wang",

year = "2000",

language = "English",

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TY - JOUR

T1 - Construction and characterization of a bacterial artificial chromosome library of peach

AU - Wang, Qian

AU - Zhang, Kaichun

AU - Qu, Xueping

AU - Jia, Jianhang

AU - Li, Chuanyou

AU - Jin, Demin

AU - Wang, Bin

PY - 2000

Y1 - 2000

N2 - This report briefly describes the construction and characterization of a peach [prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAG) library. The variety Jingyu has many important agronomic characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clones. The BAG library has an average insert size of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two randomly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach respectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and physical mapping of peach genome.

AB - This report briefly describes the construction and characterization of a peach [prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAG) library. The variety Jingyu has many important agronomic characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clones. The BAG library has an average insert size of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two randomly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach respectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and physical mapping of peach genome.

KW - Bacterial artificial chromosome

KW - Genomic library construction

KW - Prunus persica

UR - https://www.scopus.com/pages/publications/21244490883

UR - https://www.scopus.com/pages/publications/21244490883#tab=citedBy

M3 - Article

AN - SCOPUS:21244490883

SN - 1002-0071

VL - 10

SP - 526

EP - 528

JO - Progress in Natural Science

JF - Progress in Natural Science

IS - 7

ER -

Construction and characterization of a bacterial artificial chromosome library of peach

Abstract

Keywords

ASJC Scopus subject areas

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