The precise manipulation of microcirculation in mice can facilitate mechanistic studies of brain injury and repair after ischemia, but this manipulation remains a technical challenge, particularly in conscious mice. We developed a technology that uses micromagnets to induce aggregation of magnetic nanoparticles to reversibly occlude blood flow in microvessels. This allowed induction of ischemia in a specific cortical region of conscious mice of any postnatal age, including perinatal and neonatal stages, with precise spatiotemporal control but without surgical intervention of the skull or artery. When combined with longitudinal live-imaging approaches, this technology facilitated the discovery of a feature of the ischemic cascade: selective loss of smooth muscle cells in juveniles but not adults shortly after onset of ischemia and during blood reperfusion.
|Number of pages||7|
|State||Published - Feb 1 2017|
Bibliographical noteFunding Information:
Acknowledgments We thank W.Z. Sun, L.J. Wu, H. Lü, and L.J. He for advice on live imaging; J. Zheng and A. Fu for advice on MPs; M. Dellinger for advice on H.E. staining; D. Xu and H. Cai for advice on MRI; B. Zhou, I. Shimada, M. Acar, and M. Chen for input concerning SMCs, MCAO and FJC staining; W.L. Du's input for MCAO surgery; T. Taylor, H. Zhu, J.D. Chen, Z.H. Zhang, Z.P. Hu, G.E. Cai, M. Goldberg, F. Chen, L. Smith, and J. Long as well as colleagues at CRI for critical discussion and reading of the manuscript. This work is supported by the National Basic Research Program of China (No. 2015CB352006) and the Science Fund for Creative Research Group of China (No. 61121004) to W.Z.; CRI start-up funds and NINDS K99/R00 (R00NS073735) to W.-P.G.; NIH Director's New Innovator Award (DP2-NS082125) to B. Cui, American Heart Association (14SDG18410020) and NINDS (NS088555) to A.M.S.; and the Dr. Jack Krohmer Professorship in Radiation Physics for X.S. W.-P.G. is a recipient of an NINDS Pathway to Independence Award. W.L. is a recipient of an American Heart Association Postdoctoral Fellowship Award.
© 2017 Nature America, inc., part of springer Nature. All rights reserved.
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology