Controlled protein release from monodisperse biodegradable double-wall microspheres of controllable shell thickness

Yujie Xia, Pedro F. Ribeiro, Daniel W. Pack

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Biodegradable polymer microparticles are promising delivery depots for protein therapeutics due to their relatively simple fabrication and facile administration. Double-wall microspheres (DWMS) comprising a core and shell made of two distinct polymers may provide enhanced control of the drug release profiles. Using precision particle fabrication (PPF) technology, monodisperse DWMS were fabricated with model protein bovine serum albumin (BSA)-loaded poly(lactide-co-glycolide) (PLG) core and drug-free poly(d,l-lactic acid) (PDLL) shell of uniform thickness. Monolithic single-wall microspheres were also fabricated to mimic the BSA-loaded PLG core. Using ethyl acetate and dichloromethane as shell- and core-phase solvents, respectively, BSA was encapsulated selectively in the core region within DWMS with higher loading and encapsulation efficiency compared to using dichloromethane as core and shell solvents. BSA in vitro release rates were retarded by the presence of the drug-free PDLL shell. Moreover, increasing PDLL shell thickness resulted in decreasing BSA release rate. With a 14-μm thick PDLL shell, an extended period of constant-rate release was achieved.

Original languageEnglish
Pages (from-to)707-714
Number of pages8
JournalJournal of Controlled Release
Volume172
Issue number3
DOIs
StatePublished - 2013

Bibliographical note

Funding Information:
This work was supported by the National Institute of Health Grant EB005181 and GM085222 . Scanning Electron Microscopy took place in Material Research Lab at the University of Illinois at Urbana–Champaign. Confocal microscopy measurements took place in Beckman Institute, imaging technology group at the University of Illinois at Urbana–Champaign. DSC was performed in Microanalysis Laboratory, School of Chemical Sciences, University of Illinois at Urbana–Champaign.

Keywords

  • Bovine serum albumin
  • Controlled release
  • Monodisperse double-wall microspheres
  • Poly(lactic acid)
  • Poly(lactide-co-glycolide)

ASJC Scopus subject areas

  • Pharmaceutical Science

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