Corrections: Laser-Induced Protein-DNA Cross-Links via Psoralen Furanoside Monoadducts (Biochemistry (1993) 33(21) (5226–5538) (10.1021/bi00072a006))

Srinivas S. Sastry, H. Peter Spielmann, Quan Scott Hoang, A. Meleah Phillips, Aziz Sancar, John E. Hearst

Research output: Contribution to journalComment/debate

1 Scopus citations

Abstract

Page 5537. In column I, line 60, through column 2, line 2, it is implied that the difficulty in sequencing peptides that are cross-linked to nucleic acids arises from their inability to bind well to polybrene-mated supports. In fact, peptides, cross linked or not, generally bind well to this very basic support. The difficulty in sequencing such peptides is more often due to their loss prior to sequencing. In particular, cross-linked peptides often fail to elute from reverse-phase HPLC supports ludas a strong ion-pairing agent is present, and in general, these peptides seem to be relatively insoluble and/or prone to irreversible adsorption onto plastic/glass surfaca. We thank Dr. K. Williams for bringing this ambiguity to our attention.

Original languageEnglish
Pages (from-to)1616
Number of pages1
JournalBiochemistry
Volume33
Issue number6
DOIs
StatePublished - Feb 1 1994

ASJC Scopus subject areas

  • Biochemistry

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