TY - JOUR
T1 - Correlations between risk factors for breast cancer and genetic instability in cancer patients-A clinical perspective study
AU - Paz, Márcia Fernanda Correia Jardim
AU - de Alencar, Marcus Vinícius Oliveira Barros
AU - Junior, Antonio Luiz Gomes
AU - Machado, Keylla da Conceição
AU - Islam, Muhammad Torequl
AU - Ali, Eunus S.
AU - Shill, Manik Chandra
AU - Ahmed, Md Iqbal
AU - Uddin, Shaikh Jamal
AU - da Mata, Ana Maria Oliveira Ferreira
AU - de Carvalho, Ricardo Melo
AU - Machado, Kátia da Conceição
AU - Sobral, André Luiz Pinho
AU - da Silva, Felipe Cavalcanti Carneiro
AU - e Souza, João Marcelo de Castro
AU - Arcanjo, Daniel Dias Rufino
AU - Ferreira, Paulo Michel Pinheiro
AU - Mishra, Siddhartha Kumar
AU - da Silva, Juliana
AU - Melo-Cavalcante, Ana Amélia de Carvalho
N1 - Publisher Copyright:
© 2018 Paz, de Alencar, Gomes Junior, da Conceição Machado, Islam, Ali, Shill, Ahmed, Uddin, da Mata, de Carvalho, da Conceição Machado, Sobral, da Silva, de Castro e Souza, Arcanjo, Ferreira, Mishra, da Silva and de Carvalho Melo-Cavalcante.
PY - 2018/2/16
Y1 - 2018/2/16
N2 - Molecular epidemiological studies have identified several risk factors linking to the genes and external factors in the pathogenesis of breast cancer. In this sense, genetic instability caused by DNA damage and DNA repair inefficiencies are important molecular events for the diagnosis and prognosis of therapies. Therefore, the objective of this study was to analyze correlation between sociocultural, occupational, and lifestyle risk factors with levels of genetic instability in non-neoplastic cells of breast cancer patients. Total 150 individuals were included in the study that included 50 breast cancer patients submitted to chemotherapy (QT), 50 breast cancer patients submitted to radiotherapy (RT), and 50 healthy women without any cancer. Cytogenetic biomarkers for apoptosis and DNA damage were evaluated in samples of buccal epithelial and peripheral blood cells through micronuclei and comet assay tests. Elder age patients (61-80 years) had higher levels of apoptosis (catriolysis by karyolysis) and DNA damage at the diagnosis (baseline damage) with increased cell damage during QT and especially during RT. We also reported the increased frequencies of cytogenetic biomarkers in patients who were exposed to ionizing radiation as well as for alcoholism and smoking. QT and RT induced high levels of fragmentation (karyorrhexis) and nuclear dissolution (karyolysis) and DNA damage. Correlations were observed between age and karyorrhexis at diagnosis; smoking and karyolysis during RT; and radiation and karyolysis during QT. These correlations indicate that risk factors may also influence the genetic instability in non-neoplastic cells caused to the patients during cancer therapies.
AB - Molecular epidemiological studies have identified several risk factors linking to the genes and external factors in the pathogenesis of breast cancer. In this sense, genetic instability caused by DNA damage and DNA repair inefficiencies are important molecular events for the diagnosis and prognosis of therapies. Therefore, the objective of this study was to analyze correlation between sociocultural, occupational, and lifestyle risk factors with levels of genetic instability in non-neoplastic cells of breast cancer patients. Total 150 individuals were included in the study that included 50 breast cancer patients submitted to chemotherapy (QT), 50 breast cancer patients submitted to radiotherapy (RT), and 50 healthy women without any cancer. Cytogenetic biomarkers for apoptosis and DNA damage were evaluated in samples of buccal epithelial and peripheral blood cells through micronuclei and comet assay tests. Elder age patients (61-80 years) had higher levels of apoptosis (catriolysis by karyolysis) and DNA damage at the diagnosis (baseline damage) with increased cell damage during QT and especially during RT. We also reported the increased frequencies of cytogenetic biomarkers in patients who were exposed to ionizing radiation as well as for alcoholism and smoking. QT and RT induced high levels of fragmentation (karyorrhexis) and nuclear dissolution (karyolysis) and DNA damage. Correlations were observed between age and karyorrhexis at diagnosis; smoking and karyolysis during RT; and radiation and karyolysis during QT. These correlations indicate that risk factors may also influence the genetic instability in non-neoplastic cells caused to the patients during cancer therapies.
KW - Apoptosis
KW - Breast cancer
KW - Chemotherapy
KW - Genetic instability
KW - Risk factors
KW - Toxicogenomics
UR - http://www.scopus.com/inward/record.url?scp=85042564287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042564287&partnerID=8YFLogxK
U2 - 10.3389/fgene.2017.00236
DO - 10.3389/fgene.2017.00236
M3 - Article
AN - SCOPUS:85042564287
SN - 1664-8021
VL - 8
JO - Frontiers in Genetics
JF - Frontiers in Genetics
IS - FEB
M1 - 236
ER -