TY - JOUR
T1 - COVID-19 point-of-care tests can identify low-antibody individuals
T2 - In-depth immunoanalysis of boosting benefits in a healthy cohort
AU - Mallory, Michael
AU - Munt, Jennifer E.
AU - Narowski, Tara M.
AU - Castillo, Izabella
AU - Cuadra, Edwing
AU - Pisanic, Nora
AU - Fields, Paul
AU - Powers, John M.
AU - Dickson, Alexandria
AU - Harris, Rohan
AU - Wargowsky, Richard
AU - Moran, Seamus
AU - Allabban, Ahmed
AU - Raphel, Kristin
AU - McCaffrey, Timothy A.
AU - Brien, James D.
AU - Heaney, Christopher D.
AU - Lafleur, John E.
AU - Baric, Ralph S.
AU - Premkumar, Lakshmanane
N1 - Publisher Copyright:
© 2024 the Authors, some rights reserved;
PY - 2024/6
Y1 - 2024/6
N2 - The recommended COVID-19 booster vaccine uptake is low. At-home lateral flow assay (LFA) antigen tests are widely accepted for detecting infection during the pandemic. Here, we present the feasibility and potential benefits of using LFA-based antibody tests as a means for individuals to detect inadequate immunity and make informed decisions about COVID-19 booster immunization. In a health care provider cohort, we investigated the changes in the breadth and depth of humoral and T cell immune responses following mRNA vaccination and boosting in LFA-positive and LFA-negative antibody groups. We show that negative LFA antibody tests closely reflect the lack of functional humoral immunity observed in a battery of sophisticated immune assays, while positive results do not necessarily reflect adequate immunity. After booster vaccination, both groups gain depth and breadth of systemic antibodies against evolving SARS-CoV-2 and related viruses. Our findings show that LFA-based antibody tests can alert individuals about inadequate immunity against COVID-19, thereby increasing booster shots and promoting herd immunity.
AB - The recommended COVID-19 booster vaccine uptake is low. At-home lateral flow assay (LFA) antigen tests are widely accepted for detecting infection during the pandemic. Here, we present the feasibility and potential benefits of using LFA-based antibody tests as a means for individuals to detect inadequate immunity and make informed decisions about COVID-19 booster immunization. In a health care provider cohort, we investigated the changes in the breadth and depth of humoral and T cell immune responses following mRNA vaccination and boosting in LFA-positive and LFA-negative antibody groups. We show that negative LFA antibody tests closely reflect the lack of functional humoral immunity observed in a battery of sophisticated immune assays, while positive results do not necessarily reflect adequate immunity. After booster vaccination, both groups gain depth and breadth of systemic antibodies against evolving SARS-CoV-2 and related viruses. Our findings show that LFA-based antibody tests can alert individuals about inadequate immunity against COVID-19, thereby increasing booster shots and promoting herd immunity.
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U2 - 10.1126/sciadv.adi1379
DO - 10.1126/sciadv.adi1379
M3 - Article
C2 - 38865463
AN - SCOPUS:85196094613
VL - 10
JO - Science advances
JF - Science advances
IS - 24
M1 - eadi1379
ER -