CpG oligodeoxynucleotides stimulate cord blood mononuclear cells to produce immunoglobulins

Cheri D. Landers, Subbarao Bondada

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 ± 352 vs. cord 1873 ± 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 ± 58 vs. 10 ± 2.5 ng/ml) and no detectable IgA upon CpG-ODN stimulation. CpG ODN stimulated IgM production from adult CD27-negative B cells which may account for their ability to stimulate the mostly naïve cord B cells. The polyclonal IgM response included pneumococcal polysaccharide antigen-specific antibodies. CpG-ODNs may be useful as neonatal vaccine adjuvants for polysaccharide antigens that are otherwise non-immunogenic.

Original languageEnglish
Pages (from-to)236-245
Number of pages10
JournalClinical Immunology
Issue number3
StatePublished - Sep 2005

Bibliographical note

Funding Information:
This study was supported by a Children's Miracle Network Grant to Cheri Landers and NIH grants AG05731 and AI21490 to Subbarao Bondada.


  • B cell
  • CpG ODN
  • Immunoglobulins
  • Neonate
  • Pneumococcus
  • Polysaccharides

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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