CpG oligodeoxynucleotides stimulate cord blood mononuclear cells to produce immunoglobulins

Cheri D. Landers; Subbarao Bondada

doi:10.1016/j.clim.2005.04.013

CpG oligodeoxynucleotides stimulate cord blood mononuclear cells to produce immunoglobulins

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 ± 352 vs. cord 1873 ± 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 ± 58 vs. 10 ± 2.5 ng/ml) and no detectable IgA upon CpG-ODN stimulation. CpG ODN stimulated IgM production from adult CD27-negative B cells which may account for their ability to stimulate the mostly naïve cord B cells. The polyclonal IgM response included pneumococcal polysaccharide antigen-specific antibodies. CpG-ODNs may be useful as neonatal vaccine adjuvants for polysaccharide antigens that are otherwise non-immunogenic.

Original language	English
Pages (from-to)	236-245
Number of pages	10
Journal	Clinical Immunology
Volume	116
Issue number	3
DOIs	https://doi.org/10.1016/j.clim.2005.04.013
State	Published - Sep 2005

Bibliographical note

Funding Information:
This study was supported by a Children's Miracle Network Grant to Cheri Landers and NIH grants AG05731 and AI21490 to Subbarao Bondada.

Funding

This study was supported by a Children's Miracle Network Grant to Cheri Landers and NIH grants AG05731 and AI21490 to Subbarao Bondada.

Funders	Funder number
National Institutes of Health (NIH)	AG05731
National Institute of Allergy and Infectious Diseases	R01AI021490

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

B cell
CpG ODN
Immunoglobulins
Neonate
Pneumococcus
Polysaccharides

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/j.clim.2005.04.013

Cite this

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title = "CpG oligodeoxynucleotides stimulate cord blood mononuclear cells to produce immunoglobulins",

abstract = "CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 ± 352 vs. cord 1873 ± 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 ± 58 vs. 10 ± 2.5 ng/ml) and no detectable IgA upon CpG-ODN stimulation. CpG ODN stimulated IgM production from adult CD27-negative B cells which may account for their ability to stimulate the mostly na{\"i}ve cord B cells. The polyclonal IgM response included pneumococcal polysaccharide antigen-specific antibodies. CpG-ODNs may be useful as neonatal vaccine adjuvants for polysaccharide antigens that are otherwise non-immunogenic.",

keywords = "B cell, CpG ODN, Immunoglobulins, Neonate, Pneumococcus, Polysaccharides",

author = "Landers, \{Cheri D.\} and Subbarao Bondada",

note = "Funding Information: This study was supported by a Children's Miracle Network Grant to Cheri Landers and NIH grants AG05731 and AI21490 to Subbarao Bondada. ",

year = "2005",

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doi = "10.1016/j.clim.2005.04.013",

language = "English",

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AU - Landers, Cheri D.

AU - Bondada, Subbarao

N1 - Funding Information: This study was supported by a Children's Miracle Network Grant to Cheri Landers and NIH grants AG05731 and AI21490 to Subbarao Bondada.

PY - 2005/9

Y1 - 2005/9

N2 - CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 ± 352 vs. cord 1873 ± 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 ± 58 vs. 10 ± 2.5 ng/ml) and no detectable IgA upon CpG-ODN stimulation. CpG ODN stimulated IgM production from adult CD27-negative B cells which may account for their ability to stimulate the mostly naïve cord B cells. The polyclonal IgM response included pneumococcal polysaccharide antigen-specific antibodies. CpG-ODNs may be useful as neonatal vaccine adjuvants for polysaccharide antigens that are otherwise non-immunogenic.

AB - CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 ± 352 vs. cord 1873 ± 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 ± 58 vs. 10 ± 2.5 ng/ml) and no detectable IgA upon CpG-ODN stimulation. CpG ODN stimulated IgM production from adult CD27-negative B cells which may account for their ability to stimulate the mostly naïve cord B cells. The polyclonal IgM response included pneumococcal polysaccharide antigen-specific antibodies. CpG-ODNs may be useful as neonatal vaccine adjuvants for polysaccharide antigens that are otherwise non-immunogenic.

KW - B cell

KW - CpG ODN

KW - Immunoglobulins

KW - Neonate

KW - Pneumococcus

KW - Polysaccharides

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DO - 10.1016/j.clim.2005.04.013

M3 - Article

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VL - 116

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CpG oligodeoxynucleotides stimulate cord blood mononuclear cells to produce immunoglobulins

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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