TY - JOUR
T1 - CRISPR-based assays for rapid detection of SARS-CoV-2
AU - Javalkote, Vivek S.
AU - Kancharla, Nagesh
AU - Bhadra, Bhaskar
AU - Shukla, Manish
AU - Soni, Badrish
AU - Sapre, Ajit
AU - Goodin, Michael
AU - Bandyopadhyay, Anindya
AU - Dasgupta, Santanu
N1 - Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2022/7
Y1 - 2022/7
N2 - COVID-19 pandemic posed an unprecedented threat to global public health and economies. There is no effective treatment of the disease, hence, scaling up testing for rapid diagnosis of SARS-CoV-2 infected patients and quarantine them from healthy individuals is one the best strategies to curb the pandemic. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid based tests are considered to be more sensitive with respect to serological tests but present gold standard qRT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. CRISPR based novel techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here, we discuss and present compilation of state-of-the-art detection techniques for COVID-19 using CRISPR technology which has tremendous potential to transform diagnostics and epidemiology.
AB - COVID-19 pandemic posed an unprecedented threat to global public health and economies. There is no effective treatment of the disease, hence, scaling up testing for rapid diagnosis of SARS-CoV-2 infected patients and quarantine them from healthy individuals is one the best strategies to curb the pandemic. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid based tests are considered to be more sensitive with respect to serological tests but present gold standard qRT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. CRISPR based novel techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here, we discuss and present compilation of state-of-the-art detection techniques for COVID-19 using CRISPR technology which has tremendous potential to transform diagnostics and epidemiology.
KW - COVID-19
KW - CRISPR diagnostics
KW - Diagnostic advancements
KW - Molecular testing
KW - SARS-CoV-2
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U2 - 10.1016/j.ymeth.2020.10.003
DO - 10.1016/j.ymeth.2020.10.003
M3 - Review article
C2 - 33045362
AN - SCOPUS:85093671609
SN - 1046-2023
VL - 203
SP - 594
EP - 603
JO - Methods
JF - Methods
ER -