CRISPR-based assays for rapid detection of SARS-CoV-2

Vivek S. Javalkote, Nagesh Kancharla, Bhaskar Bhadra, Manish Shukla, Badrish Soni, Ajit Sapre, Michael Goodin, Anindya Bandyopadhyay, Santanu Dasgupta

Research output: Contribution to journalReview articlepeer-review

17 Scopus citations

Abstract

COVID-19 pandemic posed an unprecedented threat to global public health and economies. There is no effective treatment of the disease, hence, scaling up testing for rapid diagnosis of SARS-CoV-2 infected patients and quarantine them from healthy individuals is one the best strategies to curb the pandemic. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid based tests are considered to be more sensitive with respect to serological tests but present gold standard qRT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. CRISPR based novel techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here, we discuss and present compilation of state-of-the-art detection techniques for COVID-19 using CRISPR technology which has tremendous potential to transform diagnostics and epidemiology.

Original languageEnglish
Pages (from-to)594-603
Number of pages10
JournalMethods
Volume203
DOIs
StatePublished - Jul 2022

Bibliographical note

Funding Information:
All the figures have been created by Biorender.com. Authors also acknowledge Reliance Industries Ltd for funding.

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

  • COVID-19
  • CRISPR diagnostics
  • Diagnostic advancements
  • Molecular testing
  • SARS-CoV-2

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (all)

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