Crooked neck is a component of the human spliceosome and implicated in the splicing process

Seyung Chung; Zhaolan Zhou; Kathleen A. Huddleston; Douglas A. Harrison; Robin Reed; Timothy A. Coleman; Brian C. Rymond

doi:10.1016/S0167-4781(02)00368-8

Crooked neck is a component of the human spliceosome and implicated in the splicing process

Seyung Chung, Zhaolan Zhou, Kathleen A. Huddleston, Douglas A. Harrison, Robin Reed, Timothy A. Coleman, Brian C. Rymond

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The Drosophila crooked neck (crn) gene is essential for embryogenesis and has been implicated in cell cycle progression and in pre-mRNA splicing although a direct role in either process has not been established. Here we report isolation of the human crooked neck homolog, HCRN, and provide evidence for its function in splicing. HCRN encodes an unusual protein composed largely of tetratricopeptide repeat (TPR) elements. The crooked neck protein co-localizes with the SR and Sm protein splicing factors in discrete subnuclear domains implicated in snRNP biogenesis. In vitro assembly experiments show that an 83 kDa hcrn isoform is stably recruited to splicing complexes coincident with the addition of the U4/U6.U5 tri-snRNP particle. Crooked neck activity appears essential as extracts depleted of hcrn fail to splice pre-mRNA. These and related data support the view that crooked neck is a phylogenetically conserved pre-mRNA splicing factor.

Original language	English
Pages (from-to)	287-297
Number of pages	11
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1576
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(02)00368-8
State	Published - Jul 19 2002

Bibliographical note

Funding Information:
The authors thank Jennifer Klein and Shelly Steiner for supplying the HeLa cells used for crooked neck localization. This work was funded through grants from the National Institutes of Health to BCR and RR.

Keywords

RNA processing
Spliceosome
Splicing
Tetratricopeptide repeat

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(02)00368-8

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title = "Crooked neck is a component of the human spliceosome and implicated in the splicing process",

abstract = "The Drosophila crooked neck (crn) gene is essential for embryogenesis and has been implicated in cell cycle progression and in pre-mRNA splicing although a direct role in either process has not been established. Here we report isolation of the human crooked neck homolog, HCRN, and provide evidence for its function in splicing. HCRN encodes an unusual protein composed largely of tetratricopeptide repeat (TPR) elements. The crooked neck protein co-localizes with the SR and Sm protein splicing factors in discrete subnuclear domains implicated in snRNP biogenesis. In vitro assembly experiments show that an 83 kDa hcrn isoform is stably recruited to splicing complexes coincident with the addition of the U4/U6.U5 tri-snRNP particle. Crooked neck activity appears essential as extracts depleted of hcrn fail to splice pre-mRNA. These and related data support the view that crooked neck is a phylogenetically conserved pre-mRNA splicing factor.",

keywords = "RNA processing, Spliceosome, Splicing, Tetratricopeptide repeat",

author = "Seyung Chung and Zhaolan Zhou and Huddleston, {Kathleen A.} and Harrison, {Douglas A.} and Robin Reed and Coleman, {Timothy A.} and Rymond, {Brian C.}",

note = "Funding Information: The authors thank Jennifer Klein and Shelly Steiner for supplying the HeLa cells used for crooked neck localization. This work was funded through grants from the National Institutes of Health to BCR and RR.",

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AU - Zhou, Zhaolan

AU - Huddleston, Kathleen A.

AU - Harrison, Douglas A.

AU - Reed, Robin

AU - Coleman, Timothy A.

AU - Rymond, Brian C.

N1 - Funding Information: The authors thank Jennifer Klein and Shelly Steiner for supplying the HeLa cells used for crooked neck localization. This work was funded through grants from the National Institutes of Health to BCR and RR.

PY - 2002/7/19

Y1 - 2002/7/19

N2 - The Drosophila crooked neck (crn) gene is essential for embryogenesis and has been implicated in cell cycle progression and in pre-mRNA splicing although a direct role in either process has not been established. Here we report isolation of the human crooked neck homolog, HCRN, and provide evidence for its function in splicing. HCRN encodes an unusual protein composed largely of tetratricopeptide repeat (TPR) elements. The crooked neck protein co-localizes with the SR and Sm protein splicing factors in discrete subnuclear domains implicated in snRNP biogenesis. In vitro assembly experiments show that an 83 kDa hcrn isoform is stably recruited to splicing complexes coincident with the addition of the U4/U6.U5 tri-snRNP particle. Crooked neck activity appears essential as extracts depleted of hcrn fail to splice pre-mRNA. These and related data support the view that crooked neck is a phylogenetically conserved pre-mRNA splicing factor.

AB - The Drosophila crooked neck (crn) gene is essential for embryogenesis and has been implicated in cell cycle progression and in pre-mRNA splicing although a direct role in either process has not been established. Here we report isolation of the human crooked neck homolog, HCRN, and provide evidence for its function in splicing. HCRN encodes an unusual protein composed largely of tetratricopeptide repeat (TPR) elements. The crooked neck protein co-localizes with the SR and Sm protein splicing factors in discrete subnuclear domains implicated in snRNP biogenesis. In vitro assembly experiments show that an 83 kDa hcrn isoform is stably recruited to splicing complexes coincident with the addition of the U4/U6.U5 tri-snRNP particle. Crooked neck activity appears essential as extracts depleted of hcrn fail to splice pre-mRNA. These and related data support the view that crooked neck is a phylogenetically conserved pre-mRNA splicing factor.

KW - RNA processing

KW - Spliceosome

KW - Splicing

KW - Tetratricopeptide repeat

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Crooked neck is a component of the human spliceosome and implicated in the splicing process

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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