Cross-linked dry bonding: A new etch-and-rinse technique

Jianfeng Zhou, Ayaka Chiba, Debora L.S. Scheffel, Josimeri Hebling, Kelli Agee, Junji Tagami, Jianquo Tan, Dalia Abuelenain, Manar Abu Nawareg, Ali H. Hassan, Lorenzo Breschi, Franklin R. Tay, David H. Pashley

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Objective To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. Methods Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass% grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. Results 24 h microtensile bond strengths (μTBS) in wet and dry controls were 53.5 ± 3.6 and 9.4 ± 1.8 MPa, respectively (p < 0.05). 5% GSE in water gave μTBS of 53.7 ± 3.4 and 39.1 ± 9.7 MPa (p < 0.05), respectively, while 5% GSE in ethanol gave μTBS of 51.2 ± 2.3 and 35.3 ± 2.0 MPa (p < 0.05). 5% GSE in 5% EtOH/95% water gave wet and dry μTBS of 53.0 ± 2.3 and 55.7 ± 5.1 MPa (p > 0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p < 0.05). Significance 5% GSE pretreatment of acid-etched dentin for 1 min permits the dentin to be completely air-dried without lowering bond strength.

Original languageEnglish
Pages (from-to)1124-1132
Number of pages9
JournalDental Materials
Issue number9
StatePublished - Sep 1 2016

Bibliographical note

Publisher Copyright:
© 2016 The Academy of Dental Materials


  • Bond strength
  • Collagen degradation
  • Cross-linking
  • Dentin
  • Dentin bonding
  • Dentin stiffness
  • Dry bonding
  • Grape seed extract
  • Wet bonding

ASJC Scopus subject areas

  • General Materials Science
  • General Dentistry
  • Mechanics of Materials


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