Cross-reactive humoral and CD4+ T cell responses to Mu and Gamma SARS-CoV-2 variants in a Colombian population

Fabiola Martel, Juliana Cuervo-Rojas, Juana Ángel, Beatriz Ariza, John Mario González, Carolina Ramírez-Santana, Yeny Acosta-Ampudia, Luisa Murcia-Soriano, Norma Montoya, Claudia Cecilia Cardozo-Romero, Sandra Liliana Valderrama-Beltrán, Magda Cepeda, Julio César Castellanos, Carlos Gómez-Restrepo, Federico Perdomo-Celis, Andreu Gazquez, Alexandria Dickson, James D. Brien, José Mateus, Alba GrifoniAlessandro Sette, Daniela Weiskopf, Manuel A. Franco

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The SARS CoV-2 antibody and CD4+ T cell responses induced by natural infection and/or vaccination decline over time and cross-recognize other viral variants at different levels. However, there are few studies evaluating the levels and durability of the SARS CoV-2-specific antibody and CD4+ T cell response against the Mu, Gamma, and Delta variants. Here, we examined, in two ambispective cohorts of naturally-infected and/or vaccinated individuals, the titers of anti-RBD antibodies and the frequency of SARS-CoV-2-specific CD4+ T cells up to 6 months after the last antigen exposure. In naturally-infected individuals, the SARS-CoV-2 antibody response declined 6 months post-symptoms onset. However, the kinetic observed depended on the severity of the disease, since individuals who developed severe COVID-19 maintained the binding antibody titers. Also, there was detectable binding antibody cross-recognition for the Gamma, Mu, and Delta variants, but antibodies poorly neutralized Mu. COVID-19 vaccines induced an increase in antibody titers 15-30 days after receiving the second dose, but these levels decreased at 6 months. However, as expected, a third dose of the vaccine caused a rise in antibody titers. The dynamics of the antibody response upon vaccination depended on the previous SARS-CoV-2 exposure. Lower levels of vaccine-induced antibodies were associated with the development of breakthrough infections. Vaccination resulted in central memory spike-specific CD4+ T cell responses that cross-recognized peptides from the Gamma and Mu variants, and their duration also depended on previous SARS-CoV-2 exposure. In addition, we found cross-reactive CD4+ T cell responses in unexposed and unvaccinated individuals. These results have important implications for vaccine design for new SARS-CoV-2 variants of interest and concern.

Original languageEnglish
Article number1241038
JournalFrontiers in Immunology
Volume14
DOIs
StatePublished - 2023

Bibliographical note

Publisher Copyright:
Copyright © 2023 Martel, Cuervo-Rojas, Ángel, Ariza, González, Ramírez-Santana, Acosta-Ampudia, Murcia-Soriano, Montoya, Cardozo-Romero, Valderrama-Beltrán, Cepeda, Castellanos, Gómez-Restrepo, Perdomo-Celis, Gazquez, Dickson, Brien, Mateus, Grifoni, Sette, Weiskopf and Franco.

Keywords

  • CD4+ T cell
  • SARS-CoV-2
  • antibody
  • breakthrough infections
  • hybrid immunity
  • natural infection
  • vaccination
  • variants

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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