CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity

Hong Gu Kang; Hyong Woo Choi; Sabrina Von Einem; Patricia Manosalva; Katrin Ehlers; Po Pu Liu; Stefanie V. Buxa; Magali Moreau; Hyong Gon Mang; Pradeep Kachroo; Karl Heinz Kogel; Daniel F. Klessig

doi:10.1038/ncomms2279

CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity

Hong Gu Kang, Hyong Woo Choi, Sabrina Von Einem, Patricia Manosalva, Katrin Ehlers, Po Pu Liu, Stefanie V. Buxa, Magali Moreau, Hyong Gon Mang, Pradeep Kachroo, Karl Heinz Kogel, Daniel F. Klessig

Plant Pathology

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the ATPase and S5 domains characteristic of Microchidia (MORC) proteins; these proteins are associated with DNA modification and repair. Here we show that CRT1 and its closest homologue, CRH1, are also required for pathogen-associated molecular pattern (PAMP)-triggered immunity, basal resistance, non-host resistance and systemic acquired resistance. Consistent with its role in PAMP-triggered immunity, CRT1 interacted with the PAMP recognition receptor FLS2. Subcellular fractionation and transmission electron microscopy detected a subpopulation of CRT1 in the nucleus, whose levels increased following PAMP treatment or infection with an avirulent pathogen. These results, combined with the demonstration that CRT1 binds DNA, exhibits endonuclease activity, and affects tolerance to the DNA-damaging agent mitomycin C, argue that this prototypic eukaryotic member of the MORC superfamily has important nuclear functions during immune response activation.

Original language	English
Article number	1297
Journal	Nature Communications
Volume	3
DOIs	https://doi.org/10.1038/ncomms2279
State	Published - 2012

Bibliographical note

Funding Information:
We thank D’Maris Dempsey for critical comments on the manuscript and Euna Kim for technical support. We thank Andrew Bent for the FLS2 antibody and fls2-101, Xinnian Dong for ssn1, Shauna Somerville for pen2-2, and William Fry for P. infestans. We also thank Birgit Samans and Floyd Weckerly for advice in statistical analysis and Joseph E. Peters, Nihal Dharmasiri, Gregor Langen and Martina Claar for advice, discussion and technical support. This work was supported by grants from NSF (IOB-0641576) to D.F.K. and P.K., NSF (IOS-0820405) to D.F.K., Bundesministerium für Bildung und Forschung, Germany to K.H.K., and Texas State University-Faculty Startup Program to H.G.K.

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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@article{f87ae7dc3d324407ad803b0da9e151db,

title = "CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity",

abstract = "Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the ATPase and S5 domains characteristic of Microchidia (MORC) proteins; these proteins are associated with DNA modification and repair. Here we show that CRT1 and its closest homologue, CRH1, are also required for pathogen-associated molecular pattern (PAMP)-triggered immunity, basal resistance, non-host resistance and systemic acquired resistance. Consistent with its role in PAMP-triggered immunity, CRT1 interacted with the PAMP recognition receptor FLS2. Subcellular fractionation and transmission electron microscopy detected a subpopulation of CRT1 in the nucleus, whose levels increased following PAMP treatment or infection with an avirulent pathogen. These results, combined with the demonstration that CRT1 binds DNA, exhibits endonuclease activity, and affects tolerance to the DNA-damaging agent mitomycin C, argue that this prototypic eukaryotic member of the MORC superfamily has important nuclear functions during immune response activation.",

author = "Kang, {Hong Gu} and {Woo Choi}, Hyong and {Von Einem}, Sabrina and Patricia Manosalva and Katrin Ehlers and Liu, {Po Pu} and Buxa, {Stefanie V.} and Magali Moreau and Mang, {Hyong Gon} and Pradeep Kachroo and Kogel, {Karl Heinz} and Klessig, {Daniel F.}",

note = "Funding Information: We thank D{\textquoteright}Maris Dempsey for critical comments on the manuscript and Euna Kim for technical support. We thank Andrew Bent for the FLS2 antibody and fls2-101, Xinnian Dong for ssn1, Shauna Somerville for pen2-2, and William Fry for P. infestans. We also thank Birgit Samans and Floyd Weckerly for advice in statistical analysis and Joseph E. Peters, Nihal Dharmasiri, Gregor Langen and Martina Claar for advice, discussion and technical support. This work was supported by grants from NSF (IOB-0641576) to D.F.K. and P.K., NSF (IOS-0820405) to D.F.K., Bundesministerium f{\"u}r Bildung und Forschung, Germany to K.H.K., and Texas State University-Faculty Startup Program to H.G.K.",

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doi = "10.1038/ncomms2279",

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T1 - CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity

AU - Kang, Hong Gu

AU - Woo Choi, Hyong

AU - Von Einem, Sabrina

AU - Manosalva, Patricia

AU - Ehlers, Katrin

AU - Liu, Po Pu

AU - Buxa, Stefanie V.

AU - Moreau, Magali

AU - Mang, Hyong Gon

AU - Kachroo, Pradeep

AU - Kogel, Karl Heinz

AU - Klessig, Daniel F.

N1 - Funding Information: We thank D’Maris Dempsey for critical comments on the manuscript and Euna Kim for technical support. We thank Andrew Bent for the FLS2 antibody and fls2-101, Xinnian Dong for ssn1, Shauna Somerville for pen2-2, and William Fry for P. infestans. We also thank Birgit Samans and Floyd Weckerly for advice in statistical analysis and Joseph E. Peters, Nihal Dharmasiri, Gregor Langen and Martina Claar for advice, discussion and technical support. This work was supported by grants from NSF (IOB-0641576) to D.F.K. and P.K., NSF (IOS-0820405) to D.F.K., Bundesministerium für Bildung und Forschung, Germany to K.H.K., and Texas State University-Faculty Startup Program to H.G.K.

PY - 2012

Y1 - 2012

N2 - Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the ATPase and S5 domains characteristic of Microchidia (MORC) proteins; these proteins are associated with DNA modification and repair. Here we show that CRT1 and its closest homologue, CRH1, are also required for pathogen-associated molecular pattern (PAMP)-triggered immunity, basal resistance, non-host resistance and systemic acquired resistance. Consistent with its role in PAMP-triggered immunity, CRT1 interacted with the PAMP recognition receptor FLS2. Subcellular fractionation and transmission electron microscopy detected a subpopulation of CRT1 in the nucleus, whose levels increased following PAMP treatment or infection with an avirulent pathogen. These results, combined with the demonstration that CRT1 binds DNA, exhibits endonuclease activity, and affects tolerance to the DNA-damaging agent mitomycin C, argue that this prototypic eukaryotic member of the MORC superfamily has important nuclear functions during immune response activation.

AB - Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the ATPase and S5 domains characteristic of Microchidia (MORC) proteins; these proteins are associated with DNA modification and repair. Here we show that CRT1 and its closest homologue, CRH1, are also required for pathogen-associated molecular pattern (PAMP)-triggered immunity, basal resistance, non-host resistance and systemic acquired resistance. Consistent with its role in PAMP-triggered immunity, CRT1 interacted with the PAMP recognition receptor FLS2. Subcellular fractionation and transmission electron microscopy detected a subpopulation of CRT1 in the nucleus, whose levels increased following PAMP treatment or infection with an avirulent pathogen. These results, combined with the demonstration that CRT1 binds DNA, exhibits endonuclease activity, and affects tolerance to the DNA-damaging agent mitomycin C, argue that this prototypic eukaryotic member of the MORC superfamily has important nuclear functions during immune response activation.

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JO - Nature Communications

JF - Nature Communications

M1 - 1297

ER -

CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity

Abstract

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