@article{bd8023d25adf4724be91b76698e2c6eb,
title = "Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis",
abstract = "The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 {\AA} resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.",
keywords = "Antitumor, Biosynthesis, Carbohydrate, GT-B fold, Molecular docking, Natural product, X-ray diffraction",
author = "Fengbin Wang and Maoquan Zhou and Shanteri Singh and Yennamalli, {Ragothaman M.} and Bingman, {Craig A.} and Thorson, {Jon S.} and Phillips, {George N.}",
year = "2013",
month = jul,
doi = "10.1002/prot.24289",
language = "English",
volume = "81",
pages = "1277--1282",
number = "7",
}