TY - JOUR
T1 - Crystal structure of the N-terminal domain of EccA1 ATPase from the ESX-1 secretion system of Mycobacterium tuberculosis
AU - Wagner, Jonathan M.
AU - Evans, Timothy J.
AU - Korotkov, Konstantin V.
PY - 2014/1
Y1 - 2014/1
N2 - EccA1 is an important component of the type VII secretion system (T7SS) that is responsible for transport of virulence factors in pathogenic mycobacteria. EccA1 has an N-terminal domain of unknown function and a C-terminal AAA+ (ATPases associated with various cellular activities) domain. Here we report the crystal structure of the N-terminal domain of EccA1 from Mycobacterium tuberculosis, which shows an arrangement of six tetratricopeptide repeats that may mediate interactions of EccA1 with secreted substrates. Furthermore, the size and shape of the N-terminal domain suggest its orientation in the context of a hexamer model of full-length EccA1.
AB - EccA1 is an important component of the type VII secretion system (T7SS) that is responsible for transport of virulence factors in pathogenic mycobacteria. EccA1 has an N-terminal domain of unknown function and a C-terminal AAA+ (ATPases associated with various cellular activities) domain. Here we report the crystal structure of the N-terminal domain of EccA1 from Mycobacterium tuberculosis, which shows an arrangement of six tetratricopeptide repeats that may mediate interactions of EccA1 with secreted substrates. Furthermore, the size and shape of the N-terminal domain suggest its orientation in the context of a hexamer model of full-length EccA1.
KW - AAA+ ATPase
KW - Rv3868
KW - TPR domain
KW - Tetratricopeptide repeat
KW - Type VII secretion system
UR - http://www.scopus.com/inward/record.url?scp=84890129043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890129043&partnerID=8YFLogxK
U2 - 10.1002/prot.24351
DO - 10.1002/prot.24351
M3 - Article
C2 - 23818233
AN - SCOPUS:84890129043
SN - 0887-3585
VL - 82
SP - 159
EP - 163
JO - Proteins: Structure, Function and Bioinformatics
JF - Proteins: Structure, Function and Bioinformatics
IS - 1
ER -