TY - JOUR
T1 - Crystallization and preliminary analysis of neurolysin
AU - Lian, W.
AU - Chen, G.
AU - Wu, D.
AU - Brown, C. K.
AU - Madauss, K.
AU - Hersh, L. B.
AU - Rodgers, D. W.
PY - 2000
Y1 - 2000
N2 - Neuropeptidases inactivate or modify the activity of peptide neurotransmitters and neurohormones. The neuropeptidase neurolysin acts only on short peptides and accepts a variety of cleavage-site sequences. Structures of the enzyme and enzyme-substrate complexes will help to determine the mechanisms of substrate selectivity used by this enzyme. Crystals of recombinant neurolysin have been grown in the orthorhombic space group P21212, with unit-cell parameters a = 157.8, b = 88.0, c = 58.4 Å. Data have been collected to 2.3 Å at 110 K with observed diffraction to 1.8 Å. Circular dichroism measurements suggest that the enzyme is primarily α-helical, with little β-strand secondary structure. Sequence-based secondary-structure prediction supports this conclusion.
AB - Neuropeptidases inactivate or modify the activity of peptide neurotransmitters and neurohormones. The neuropeptidase neurolysin acts only on short peptides and accepts a variety of cleavage-site sequences. Structures of the enzyme and enzyme-substrate complexes will help to determine the mechanisms of substrate selectivity used by this enzyme. Crystals of recombinant neurolysin have been grown in the orthorhombic space group P21212, with unit-cell parameters a = 157.8, b = 88.0, c = 58.4 Å. Data have been collected to 2.3 Å at 110 K with observed diffraction to 1.8 Å. Circular dichroism measurements suggest that the enzyme is primarily α-helical, with little β-strand secondary structure. Sequence-based secondary-structure prediction supports this conclusion.
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U2 - 10.1107/S0907444900012683
DO - 10.1107/S0907444900012683
M3 - Article
C2 - 11092934
AN - SCOPUS:0034517596
SN - 0907-4449
VL - 56
SP - 1644
EP - 1646
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 12
ER -