TY - JOUR
T1 - Crystallization and preliminary X-ray characterization of the nitrile reductase QueF
T2 - A queuosine-biosynthesis enzyme
AU - Swairjo, Manal A.
AU - Reddy, Robert R.
AU - Lee, Bobby
AU - Van Lanen, Steven G.
AU - Brown, Shannon
AU - De Crécy-Lagard, Valérie
AU - Iwata-Reuyl, Dirk
AU - Schimmel, Paul
PY - 2005
Y1 - 2005
N2 - QueF (MW = 19.4 kDa) is a recently characterized nitrile oxidoreductase which catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine, a late step in the biosynthesis of the modified tRNA nucleoside queuosine. Initial crystals of homododecameric Bacillus subtilis QueF diffracted poorly to 8.0 Å. A three-dimensional model based on homology with the tunnel-fold enzyme GTP cyclohydrolase I suggested catalysis at intersubunit interfaces and a potential role for substrate binding in quaternary structure stabilization. Guided by this insight, a second crystal form was grown that was strictly dependent on the presence of preQ0. This crystal form diffracted to 2.25 Å resolution.
AB - QueF (MW = 19.4 kDa) is a recently characterized nitrile oxidoreductase which catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine, a late step in the biosynthesis of the modified tRNA nucleoside queuosine. Initial crystals of homododecameric Bacillus subtilis QueF diffracted poorly to 8.0 Å. A three-dimensional model based on homology with the tunnel-fold enzyme GTP cyclohydrolase I suggested catalysis at intersubunit interfaces and a potential role for substrate binding in quaternary structure stabilization. Guided by this insight, a second crystal form was grown that was strictly dependent on the presence of preQ0. This crystal form diffracted to 2.25 Å resolution.
UR - https://www.scopus.com/pages/publications/33744489086
UR - https://www.scopus.com/pages/publications/33744489086#tab=citedBy
U2 - 10.1107/S1744309105029246
DO - 10.1107/S1744309105029246
M3 - Article
C2 - 16511203
AN - SCOPUS:33744489086
SN - 1744-3091
VL - 61
SP - 945
EP - 948
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 10
ER -