Cycling cross-bridges increase myocardial stiffness at submaximal levels of Ca2+ activation

Kenneth S. Campbell, Jitandrakumar R. Patel, Richard L. Moss

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Permeabilized multicellular preparations of canine myocardium were subjected to controlled length changes to investigate the extent to which cross-bridges augment passive stiffness components in myocardium at low levels of Ca2+ activation. When the preparations were immersed in pCa 9.0 solution (negligible free [Ca2+]) they behaved as simple elastic systems (i.e., tension increased proportionately with length). In contrast, when the muscles were stretched in Ca2+ activating solutions, tension rose much more rapidly during the initial phase of the movement than thereafter. Several lines of evidence suggest that the nonlinear response represents the displacement of populations of cycling cross-bridges that are perturbed by interfilamentary movement and take some time to recover. 1), The stiffness of the initial phase increased proportionately with the level of Ca2+ activation. 2), The magnitude of the short-range response increased with stretch velocity. 3), The initial response was reversibly reduced by 5-mM 2,3-butanedione monoxime, a known cross-bridge inhibitor. The initial stiffness of the passive elastic (pCa 9.0) response was equivalent to the Ca2+ dependent component at 2% (pCa ∼ 6.2) of the maximal (pCa 4.5) level. These results suggest that cross-bridges may significantly affect diastolic chamber stiffness.

Original languageEnglish
Pages (from-to)3807-3815
Number of pages9
JournalBiophysical Journal
Volume84
Issue number6
DOIs
StatePublished - Jun 1 2003

Bibliographical note

Funding Information:
This work was supported by grants from the American Heart Association and the National Institutes of Health (AHA 0120574Z to K.S.C. and HL47053 to R.L.M.). K.S.C. is a Postdoctoral Fellow of the Northland Affiliate of the American Heart Association.

ASJC Scopus subject areas

  • Biophysics

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