Cys18-Cys137 disulfide bond in mouse angiotensinogen does not affect AngII-dependent functions in vivo

Congqing Wu, Yinchuan Xu, Hong Lu, Deborah A. Howatt, Anju Balakrishnan, Jessica J. Moorleghen, Craig W.Vander Kooi, Lisa A. Cassis, Jian An Wang, Alan Daugherty

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Renin cleavage of angiotensinogen (AGT) releases angiotensin I (AngI) in the initial step of producing all angiotensin peptides. It has been suggested recently that redox regulation of a disulfide bond in AGT involving Cys18-Cys137 may be important to its renin cleavage efficiency in vivo. The purpose of this study was to test this prediction in a mouse model by comparing AngII production and AngII-dependent functions in mice expressing wild-type AGT versus a mutated form of AGT lacking the disulfide bond. Wild-type (hepAGT+/+) and hepatocyte-specific AGT-deficient (hepAGT-/-) littermates were developed in an low-density lipoprotein receptor -/- background. hepAGT+/+ mice were injected intraperitoneally with adeno-associated viral (AAV) vector containing a null insert. hepAGT-/- mice were injected with AAV containing a null insert, wild-type AGT or Cys18Ser and Cys137Ser mutated AGT. Two weeks after AAV injection, mice were fed a Western diet for 12 weeks. Administration of AAV containing either form of AGT led to similar plasma AGT concentrations in hepAGT-/- mice. High plasma renin concentrations in hepAGT-/- mice were suppressed equally by both forms of AGT, which were accompanied by comparable increases of plasma AngII concentrations similar to hepAGT+/+ mice. AAV-driven expression of both forms of AGT led to equivalent increases of systolic blood pressure and augmentation of atherosclerotic lesion size in hepAGT-/- mice. These measurements were comparable to systolic blood pressure and atherosclerotic lesions in hepAGT+/+ mice. These data indicate that the Cys18-Cys137 disulfide bond in AGT is dispensable for AngII production and AngII-dependent functions in mice.

Original languageEnglish
Pages (from-to)800-805
Number of pages6
JournalHypertension
Volume65
Issue number4
DOIs
StatePublished - Apr 20 2015

Bibliographical note

Publisher Copyright:
© 2015 American Heart Association, Inc.

Keywords

  • angiotensin
  • angiotensinogen
  • atherosclerosis
  • blood pressure

ASJC Scopus subject areas

  • Internal Medicine

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