Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay

Joao A. Paulo; Linda S. Lee; Bechien Wu; Peter A. Banks; Hanno Steen; Darwin L. Conwell

doi:10.1016/j.jim.2011.04.012

Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay

Joao A. Paulo, Linda S. Lee, Bechien Wu, Peter A. Banks, Hanno Steen, Darwin L. Conwell

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method, (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meq/L] of the CP and CAP patients were 43 and 97, respectively. Statistically significant decreases in the cytokine concentrations of EGF, IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens (p. <. 0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis.

Original language	English
Pages (from-to)	98-107
Number of pages	10
Journal	Journal of Immunological Methods
Volume	369
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2011.04.012
State	Published - Jun 30 2011

Bibliographical note

Funding Information:
Funds were provided by the following NIH grants: 1 F32 DK085835-01A1 (JP), 1 R21 DK081703-01A2 (DC) and 5 P30 DK034854-24 (Harvard Digestive Diseases Center; DC). In addition, we would like to thank the Burrill family for their generous support through the Burrill Research Grant. We would also like to thank members of the Steen Laboratory at Children's Hospital Boston and Harvard Medical School, in particular John FK Sauld and Robert Everley for their technical assistance and critical reading of the manuscript.

Keywords

Biomarker
EPFT
Pancreas
Pancreas fluid
Pancreatic juice
Pancreatitis

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jim.2011.04.012

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@article{0fcf861ae1534e298e2dcdeade9d41e0,

title = "Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay",

abstract = "Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method, (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meq/L] of the CP and CAP patients were 43 and 97, respectively. Statistically significant decreases in the cytokine concentrations of EGF, IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens (p. <. 0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis.",

keywords = "Biomarker, EPFT, Pancreas, Pancreas fluid, Pancreatic juice, Pancreatitis",

author = "Paulo, {Joao A.} and Lee, {Linda S.} and Bechien Wu and Banks, {Peter A.} and Hanno Steen and Conwell, {Darwin L.}",

note = "Funding Information: Funds were provided by the following NIH grants: 1 F32 DK085835-01A1 (JP), 1 R21 DK081703-01A2 (DC) and 5 P30 DK034854-24 (Harvard Digestive Diseases Center; DC). In addition, we would like to thank the Burrill family for their generous support through the Burrill Research Grant. We would also like to thank members of the Steen Laboratory at Children's Hospital Boston and Harvard Medical School, in particular John FK Sauld and Robert Everley for their technical assistance and critical reading of the manuscript.",

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AU - Paulo, Joao A.

AU - Lee, Linda S.

AU - Wu, Bechien

AU - Banks, Peter A.

AU - Steen, Hanno

AU - Conwell, Darwin L.

N1 - Funding Information: Funds were provided by the following NIH grants: 1 F32 DK085835-01A1 (JP), 1 R21 DK081703-01A2 (DC) and 5 P30 DK034854-24 (Harvard Digestive Diseases Center; DC). In addition, we would like to thank the Burrill family for their generous support through the Burrill Research Grant. We would also like to thank members of the Steen Laboratory at Children's Hospital Boston and Harvard Medical School, in particular John FK Sauld and Robert Everley for their technical assistance and critical reading of the manuscript.

PY - 2011/6/30

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N2 - Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method, (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meq/L] of the CP and CAP patients were 43 and 97, respectively. Statistically significant decreases in the cytokine concentrations of EGF, IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens (p. <. 0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis.

AB - Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method, (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meq/L] of the CP and CAP patients were 43 and 97, respectively. Statistically significant decreases in the cytokine concentrations of EGF, IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens (p. <. 0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis.

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KW - Pancreas

KW - Pancreas fluid

KW - Pancreatic juice

KW - Pancreatitis

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SN - 0022-1759

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JO - Journal of Immunological Methods

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IS - 1-2

ER -

Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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