Cytokine response patterns to complex biofilms by mononuclear cells discriminate patient disease status and biofilm dysbiosis

I. M. Velsko, Y. Cruz-Almeida, H. Huang, S. M. Wallet, L. M. Shaddox

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Localized aggressive periodontitis (LAP) is a rare form of periodontal disease with site-specific rapid tissue destruction. A lipopolysaccharide (LPS) hyper-inflammatory response was shown in LAP using peripheral whole blood, although responses to other bacterial surface components or complex oral biofilms have not been evaluated. Peripheral blood mononuclear cells (PBMCs) from 14 LAP patients, 15 healthy siblings (HS), and 13 unrelated healthy controls (HC) were stimulated with: LPS, lipoteichoic acid, or peptidoglycan; intact or sonically dispersed in vitro–grown biofilms from a LAP disease site, a LAP healthy site, or a healthy control site. Cell culture supernatants were assayed for 14 cyto/chemokines. Discriminant function analysis determined cyto/chemokines that discriminate disease status by response patterns to different stimuli. Qualitative differences in the cytokine response pattern among patient groups were observed to intact and dispersed biofilms, yet responses to healthy and diseased biofilms could not be discriminated. Despite an equivalent magnitude of response, LAP-derived PBMCs demonstrated a qualitatively different pattern of response to LPS and dispersed biofilms. PMBCs from each group responded distinctly to stimulation withsubgingival biofilms. Multiple underlying mechanisms related to bacterial-induced inflammatory responses can culminate in LAP disease initiation and/or progression, and biofilm homeostasis could play an important role.

Original languageEnglish
Article number1330645
JournalJournal of Oral Microbiology
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2017

Bibliographical note

Funding Information:
This work was supported by NIH NIDCR R01DE019456 (L.M.S.) and NIH NIDCR T90 DE021990 (I.M.V.). We thank Dr. Ann Progulske-Fox for the use of her anaerobic chamber, and Wesley Dickerson for isolation of patient PBMCs.

Publisher Copyright:
© 2017, © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • Periodontal disease
  • biofilm homeostasis
  • cytokine
  • host–pathogen interaction
  • inflammatory response
  • localized aggressive periodontitis
  • subgingival biofilm

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Microbiology (medical)
  • Infectious Diseases

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