Abstract
Pawpaw is native to Eastern North America and shows good potential as a tree fruit crop. Micropropagation could provide a clonal propagation method for plant production and germplasm preservation. Once established, pawpaw cultures typically produce many shoot-bud clusters that do not readily elongate. Shoot-bud cultures that had been maintained on a BA (8.9 μM) + NAA (2.3 μM) medium for over five years showed evidence of cytokinin habituation. Single shoot-buds (1.5 cm) moved to a media with or without plant growth regulators (PGR) continued to initiate new shoots at a similar rate (~ 5 to 8 shoots per culture). Shoots on PGR medium failed to elongate shoots greater than 2 cm compared to approximately 12% for those on PGR-free medium. Single shoot-buds subcultured three times to PGR-free medium continued to initiate between 5 to 7 shoot-buds per culture and shoot elongation remained at approximately 16%. This sensitivity to cytokinin and apparent carryover effect may in part explain the recalcitrant nature of pawpaw microcuttings to rooting.
Original language | English |
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Pages (from-to) | 371-374 |
Number of pages | 4 |
Journal | Acta Horticulturae |
Volume | 738 |
DOIs | |
State | Published - 2007 |
Keywords
- Asimina
- Autotrophic
- Micropropagation
- Shoot formation
- Tissue culture
ASJC Scopus subject areas
- Horticulture