Cytokinin habituation for autonomous shoot initiation in pawpaw (asimina triloba)

Robert L. Geneve, Sharon T. Kester, Kirk W. Pomper

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Pawpaw is native to Eastern North America and shows good potential as a tree fruit crop. Micropropagation could provide a clonal propagation method for plant production and germplasm preservation. Once established, pawpaw cultures typically produce many shoot-bud clusters that do not readily elongate. Shoot-bud cultures that had been maintained on a BA (8.9 μM) + NAA (2.3 μM) medium for over five years showed evidence of cytokinin habituation. Single shoot-buds (1.5 cm) moved to a media with or without plant growth regulators (PGR) continued to initiate new shoots at a similar rate (~ 5 to 8 shoots per culture). Shoots on PGR medium failed to elongate shoots greater than 2 cm compared to approximately 12% for those on PGR-free medium. Single shoot-buds subcultured three times to PGR-free medium continued to initiate between 5 to 7 shoot-buds per culture and shoot elongation remained at approximately 16%. This sensitivity to cytokinin and apparent carryover effect may in part explain the recalcitrant nature of pawpaw microcuttings to rooting.

Original languageEnglish
Pages (from-to)371-374
Number of pages4
JournalActa Horticulturae
StatePublished - 2007


  • Asimina
  • Autotrophic
  • Micropropagation
  • Shoot formation
  • Tissue culture

ASJC Scopus subject areas

  • Horticulture


Dive into the research topics of 'Cytokinin habituation for autonomous shoot initiation in pawpaw (asimina triloba)'. Together they form a unique fingerprint.

Cite this