TY - JOUR
T1 - Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates
T2 - Detection and analysis by PCR
AU - Eyigor, Aysegul
AU - Dawson, Karl A.
AU - Langlois, Bruce E.
AU - Pickett, Carol L.
PY - 1999
Y1 - 1999
N2 - Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently, it may be possible to speciate C. jejuni and C. coli isolates on the basis of restriction patterns within their cdt genes.
AB - Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently, it may be possible to speciate C. jejuni and C. coli isolates on the basis of restriction patterns within their cdt genes.
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U2 - 10.1128/jcm.37.5.1646-1650.1999
DO - 10.1128/jcm.37.5.1646-1650.1999
M3 - Article
C2 - 10203548
AN - SCOPUS:0032895280
SN - 0095-1137
VL - 37
SP - 1646
EP - 1650
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -