Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: Detection and analysis by PCR

Aysegul Eyigor, Karl A. Dawson, Bruce E. Langlois, Carol L. Pickett

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently, it may be possible to speciate C. jejuni and C. coli isolates on the basis of restriction patterns within their cdt genes.

Original languageEnglish
Pages (from-to)1646-1650
Number of pages5
JournalJournal of Clinical Microbiology
Volume37
Issue number5
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Microbiology (medical)

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